LncRNA UCA1 siRNA, CXCR4 siRNA, and miR-204 mimics were purchased from GenePharma (Shanghai, China). UCA1 was amplified from the cDNA of PC3 cells using PrimerSTAR (TaKaRa) and cloned into the pcDNA3.1(+) vector. All cells were transfected with 100 nM miR-204 mimics, UCA1 siRNA, CXCR4 siRNA, or 2 µg pcDNA3.1(+)-UCA1 expression vector using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions. The WT and MT 3′UTR of CXCR4 or the UCA1 fragment containing the miR-204 binding sites were synthesized and then cloned into the luciferase reporter vector p-Luc.
Cxcr4 sirna
Manufactured by GenePharma
Sourced in China
CXCR4 siRNA is a type of small interfering RNA (siRNA) that targets the CXCR4 gene. The CXCR4 gene encodes a chemokine receptor that plays a role in various cellular processes. The CXCR4 siRNA can be used to knockdown the expression of the CXCR4 gene in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Primerstar, by Takara Bio (1 mentions)
Cxcr4 sirna, by Thermo Fisher Scientific (1 mentions)
Mir 204 mimic, by GenePharma (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Non targeting control sirna, by GenePharma (1 mentions)
2 protocols using cxcr4 sirna
1
Modulating CXCR4 and UCA1 in Cancer
2
CXCR4 siRNA Transfection in H1299 Cells
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The CXCR4 siRNA or non-targeting control siRNA were synthesized by GenePharma (Shanghai, China). H1299 cells at 70-80% confluence were transfected with siRNAs using Lipofectamine RNAiMAX reagent (Invitrogen, USA) according to the manufacturer's protocol. At 6 h after transfection, the medium was changed to RPMI 1640 containing 10% FBS.
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