Ten amino acids (cystine, isoleucine, proline, threonine, phenylalanine, histidine, beta-alanine, ornithine, glutamic acid, and tyrosine) were analyzed in untargeted GC-MS datasets measured on 14 participants in the study (7 in the NW group and 7 in the OB group) to complement the panel of metabolites identified by LC-MS. The samples were prepared according to the procedure of the Human Serum Metabolome Consortium [78 (link)] and measured on an Agilent 7890B/5977 A GC-MS system, 70 eV, equipped with a DB-5MS column 60 m × 0.250 mm × 0.25 μm (Agilent Technologies, Santa Clara, CA, USA) as detailed in Pimentel et al. [79 (link)]. The data for each amino acid was analyzed with Agilent MassHunter (Agilent Technologies, V.B.7.00, V.B.8.00) using U13C6-labeled D-Fructose (Cambridge Isotope Laboratories, Inc., Tewksburg, MA, USA) as an internal standard to correct the peak areas of the amino acids prior to statistical evaluation. The statistical analyses of the amino acids measured by GC-MS were conducted as for the LC-MS data, except that it was not corrected for multiple testing.
Agilent 7890b 5977a gc ms system
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890B/5977A GC-MS system is a gas chromatography-mass spectrometry (GC-MS) instrument designed for analytical applications. The system combines a 7890B gas chromatograph and a 5977A mass spectrometer, providing comprehensive analytical capabilities for identifying and quantifying chemical compounds.
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4 protocols using agilent 7890b 5977a gc ms system
1
Untargeted GC-MS Analysis of Amino Acids
2
OSHA and NIOSH Modified GC/MS Protocols for Volatile Carbonyls
3
Extraction and Analysis of Stenochlaena tuberculata Essential Oil
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Fresh samples of S. tuberculata (250 g) were soaked in water (3,000 mL) for 12 h, and then distilled at the temperature of 200°C for 10 h using a Clevenger-type apparatus.22 The yellow-colored STO was collected and stored in sealed vials at 4°C. The compositional analysis of STO was performed using an Agilent 7890B/5977A GC-MS system (Agilent Technologies, Santa Clara, CA, USA) equipped with an HP-5MS capillary column (30×0.25 mm, 0.25 μm film thickness; Agilent Technologies). STO was diluted in ether at a ratio of 1:250 (v/v). A split of 1:15 (v/v) was used for the injection of 1 μL of the sample. The injector temperature was 300°C, and the gas chromatography (GC) oven was programmed from 100°C to 280°C at 10°C/min. High-purity helium was used as carrier gas at a flow rate of 1.2 mL/min. The ionization voltage was 70 eV, and the ion source was kept at 230°C. The chemical constituents were identified by comparing the spectra with the mass spectrometry (MS) library database (NIST 11). Component relative amounts were calculated based on GC peak areas without any correction factor.
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GC-MS Metabolite Profiling Protocol
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Samples dissolved in pyridine were incubated at 37 °C for 1.5 h and silanized for 1 h at 37 °C with the addition of 80 μL N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA). The reaction was then stopped by adding 10 μL hexane into the samples. The derivative sample was auto-injected into an Agilent 7890B/5977A GC-MS system (Agilent, San Jose, CA, USA), which was equipped with a DB-5MS column (length 30 m, i.d. 0.25 mm; Agilent), and the injection temperature was held at 300 °C. The oven temperature program was set as follows: initially kept at 60 °C for 3 min, 5 °C·min−1 to 170 °C, 4 °C·min−1 to 234 °C, 5 °C·min−1 to 270 °C, then heated to 300 °C at 10 °C·min−1, and held for 5 min [10 (link)]. The voltage of the detector was set to 0.93 kV, and the EI ionization voltage of the metabolites was 70 eV. Mass spectra were recorded from m/z 50 to m/z 450. Peaks were identified through spectral matching against standards from the National Institute of Standards and Technology (NIST) standard database, and a relative score greater than 700 was considered to be a good match.
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