Anti p pka c

B cells cultured under various conditions were harvested and cell lysates were prepared in lysis buffer. The lysate was resolved on a 10% reducing SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride membrane. After blocking with Tris-buffered saline (pH 7.4) containing 5% dried skimmed milk, the membrane was incubated with antibodies, followed by probing with HRP-conjugated anti-rabbit or -mouse antibody (Sigma). The bands were detected by chemiluminescence with ECL detection reagents (Life Technologies). Antibody to Phospho-STAT6 (Tyr641, 56,554), anti-STAT6 (5,397), anti-p-PKA C(4,781), anti-PKA C-α (5,842), anti-p-CREB (9,198), anti-CREB (9,197), PI3 Kinase p110 δ 34,050, anti-p-Akt (Ser473, 4,060), anti-Akt (4,685), anti-p-FoxO1 (Ser256, 9,461), anti-FoxO1 (2,880), anti-p-FoxO3a (Ser253, 13,129), anti-FoxO3a (12,829), anti-PPARγ (2,443) were from Cell Signaling Technology (Danvers, MA, USA). Anti-ubiquitin (PTM-1106) was from PTM BIO (Beijing, China). Anti-β-Actin (66009-1-Ig) was from Proteintech.

The Entinostat (MS-275) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). The LD1 and BLT were provided by Prof. Hwan Myung Kim, which showed low cytotoxicity to allow live sample imaging, as confirmed by the previous study [54 (link),55 (link)]. The CF405M-conjugated wheat germ agglutinin (WGA) was purchased from Biotium (Fremont, CA, USA). Anti-p-AMPK (#4185S), anti-AMPK (#4150), anti-p-PKA C (#4781S), anti-PKA C-α (#4782), anti-SQSTM1/p62 (#5114S), and anti-LC3B (#2775S) were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin (#A300-491A) and anti-FGF21 (#PA5-79254) were purchased from Bethyl Laboratories (Montgomery, TX, USA) and Thermo Fisher Scientific (Waltham, MA, USA), respectively.