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Oct freezing medium

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The OCT freezing medium is a laboratory product used to prepare tissue samples for cryosectioning in optical coherence tomography (OCT) analysis. It is a colorless, water-soluble liquid designed to provide optimal freezing and embedding conditions for the specimen.

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OCT medium (7 citations) OCT tissue freezing medium (7 citations) Leica OCT cryocompound tissue freezing medium (2 citations)

3 protocols using oct freezing medium

1

Foreskin Tissue Collection and Processing

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Circumcision was performed using the dorsal slit method either with or without the aid of a clamp and foreskins were shipped within 24 h to the laboratories in Cape Town for processing. Tissues were separated into inner and outer foreskins. Smaller, 5–7 mm2-sized sections were excised and snap-frozen in self-made foil cryomolds containing optimal cutting temperature (OCT) freezing medium (Leica Biosystems, Nussloch, Germany). Tissue samples used for the chemokine gene array were stored and frozen in RPMI with 10% dimethyl sulfoxide (DMSO). In addition, first-pass urine and penile swabs were collected for STI analysis.
Gray C.M., O’Hagan K.L., Lorenzo-Redondo R., Olivier A.J., Amu S., Chigorimbo-Murefu N., Harryparsad R., Sebaa S., Maziya L., Dietrich J., Otwombe K., Martinson N., Ferrian S., Mkhize N.N., Lewis D.A., Lang D., Carias A.M., Jaspan H.B., Wilson D.P., McGilvray M., Cianci G.C., Anderson M.R., Dinh M.H., Williamson A.L., Passmore J.A., Chiodi F, & Hope T.J. (2019). Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males. Mucosal Immunology, 13(1), 118-127.
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2

Foreskin Tissue Collection and Processing

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Circumcision was performed using the dorsal slit method either with or without the aid of a clamp and foreskins were shipped within 24 hours to the laboratories in Cape Town for processing. Tissues were separated into inner and outer foreskins. Smaller, 5–7 mm2 sized sections were excised and snap-frozen in self-made foil cryomolds containing optimal cutting temperature (OCT) freezing medium (Leica Biosystems, Nussloch, Germany). Tissue samples used for the chemokine gene array were stored and frozen in RPMI with 10% dimethyl sulfoxide (DMSO). Additionally first-pass urine and penile swabs were collected for STI analysis.
Gray C.M., O’Hagan K.L., Lorenzo-Redondo R., Olivier A.J., Amu S., Chigorimbo-Murefu N., Harryparsad R., Sebaa S., Maziya L., Dietrich J., Otwombe K., Martinson N., Ferrian S., Mkhize N.N., Lewis D.A., Lang D., Carias A.M., Jaspan H.B., Wilson D.P., McGilvray M., Cianci G.C., Anderson M.R., Dinh M.H., Williamson A.L., Passmore J.A., Chiodi F, & Hope T.J. (2019). Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males. Mucosal Immunology, 13(1), 118-127.
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3

Histological Analysis of Murine Hearts

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For general histological analysis, whole hearts were fixed in 4% v/v paraformaldehyde (PFA)/PBS for 48 h at RT under gentle agitation and dehydrated in an increasing ethanol gradient, embedded in paraffin and cut into 4μm sections. For analysis of endogenous fluorescence in the αMHC-MerCreMer-Confetti mice, hearts were briefly fixed in 4% v/v PFA solution for 4 h at 4°C under gentle agitation. Hearts were rinsed in PBS and immersed in 30% w/v sucrose solution (prepared in PBS). Once hearts had sunk to the bottom of the tube (∼24 h), hearts were embedded in optimal cutting temperature (OCT) freezing medium (Leica Biosystems, The Netherlands) and flash frozen.
Gladka M.M., Johansen A.K., van Kampen S.J., Peters M.M., Molenaar B., Versteeg D., Kooijman L., Zentilin L., Giacca M, & van Rooij E. (2022). Thymosin β4 and prothymosin α promote cardiac regeneration post-ischaemic injury in mice. Cardiovascular Research, 119(3), 802-812.
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