B16F10 murine and SK-MEL-28 human melanoma cell lineswere purchased from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in Dulbecco's modified Eagle's medium (B16F10 cells) or modified Eagle's medium (SK-MEL-29 cells, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Atlas Biologicals, For Collins, Co, USA), 100 U/ml penicillin and 100 μg streptomycin (Sigma-Aldrich). Human umbilical vein endothelial cells (HUVECs) and their growth medium (Endothelial Cell Growth Medium) were purchased from PromoCell GmbH (Heidelberg, Germany). The cells were used at passage 4-5 in all experiments. All cells were incubated at 37 °C with 5% CO2. Naringenin was dissolved in dimethyl sulfoxide to obtain a 200 μM stock solution, which was then diluted with media.
B16f10 murine
Manufactured by Korean Cell Line Bank
The B16F10 murine cell line is a well-characterized and widely used melanoma cell line derived from C57BL/6 mice. It is a highly metastatic and aggressive tumor cell line commonly used in cancer research and drug development.
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Lab products found in correlation
B16f10 cells, by Thermo Fisher Scientific (1 mentions)
Sk mel 28, by Korean Cell Line Bank (1 mentions)
Human umbilical vein endothelial cells huvecs, by PromoCell (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions)
Endothelial cell growth medium, by PromoCell (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
2 protocols using b16f10 murine
1
Melanoma and HUVEC Cell Culture Protocol
2
Evaluating Antimelanogenic Effects of CJEFs
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Cellular assays were conducted to evaluate the antimelanogenic effects of CJEFs in B16F10 murine melanoma cells that were purchased from Korea Cell Line Bank (Seoul, Korea). To mimic UV-induced overproduction of melanin, cells were stimulated with 100 nM α-MSH. A separate blank group was also cultured as a negative control without α-MSH stimulation and CJEF treatment. The cells were propagated in T-25 cell culture flasks before seeding into 96- or 6-well plates for assays. The cells were fed with Dulbecco’s modified Eagle’s medium (Welgene, Gyeongsan, Korea) containing 10% fetal bovine serum (Welgene) and 1% L-glutamine penicillin-streptomycin solution (Welgene). Between experiments, the cells were placed in incubators at 37°C and 5% CO2.
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