FoxM1 inhibitor, thiostrepton was purchased from Tocris Cookson Inc (Ellisville, MO). β-Catenin inhibitor, FH535 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against β-catenin (CST-9562), cMYC (CST-5605), MMP-9 (CST-2270), MMP-2 (CST-4022), caspase-3 (CST-9665), Bcl-2 (CST-2876) and Bcl-xL (CST-2762), β-actin (CST-3700) were purchased from Cell Signaling Technology (Beverly MA). Anti–active-β-catenin (clone 8E7#05-665) antibody was purchased from EMD Millipore (Billerica, MA). FoxM1 (sc-502), TCF4 (sc-166699), CyclinD1 (sc-753), uPAR (sc-10815), VEGF (sc-57496), Survivin (sc-374616), PARP (sc-7150) and GAPDH (sc-25778) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Annexin V was purchased from Molecular Probes (Eugene, OR).
Thiostrepton
Manufactured by Bio-Techne
Sourced in Macao
Thiostrepton is a natural product isolated from certain streptomycetes. It functions as an antibiotic and exhibits inhibitory activity against several gram-positive bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5, by Thermo Fisher Scientific (1 mentions)
Fh535, by Merck Group (1 mentions)
Anti active β catenin clone 8e7 05 665 antibody, by Merck Group (1 mentions)
Gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
293t cell line, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using thiostrepton
1
Investigating Oncogenic Pathways Modulation
2
Nasopharyngeal Cancer Cell Lines: Cultivation and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell lines and reagents. Human nasopharyngeal cancer cell lines, CNE-1 and CNE-2, purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China) were maintained in RPMI-1640 medium supplemented with 10% newborn calf serum (NBCS), 100 u/ml penicillin and 100 µg/ml streptomycin at 37˚C in a humidified atmosphere of 5% CO 2 and 95% air. The 293T cell line was obtained from Invitrogen (Carlsbad, CA, uSA) and cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS; Invitrogen), 100 u/ml penicillin and 100 µg/ml streptomycin. Thiostrepton is a cycling polypeptide antibiotic with selective inhibitory activity against FoxM1 (23) and was purchased from Tocris Cookson (Ellisville, MO, uSA). Antibodies against FoxM1, E-cadherin, zinc finger E-box binding homeobox 2 (ZEB2), zinc finger protein Snail2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, uSA).
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