Caspase 3 activity was determined using a Caspase Activity Assay Kit (Sigma-Aldrich, Burlington, USA). The activity was measured according to the cleavage of DEVD-p-nitroanilide to yellow-colored p-nitroaniline through time [26 (link)]. The caspase 3 activity was calculated in µmol p-nitroaniline released per min per ml of cell lysate. All samples were tested as triplicates and normalized to normal control.
Caspase activity assay kit
Manufactured by Merck Group
Sourced in United States
The Caspase Activity Assay Kit is a laboratory tool designed to measure the activity of caspase enzymes, which play a key role in programmed cell death (apoptosis). The kit provides a simple and sensitive fluorometric method for detecting the activation of various caspase subtypes. It allows for the quantitative analysis of caspase activity in cell lysates or purified enzyme preparations.
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Lab products found in correlation
2 protocols using caspase activity assay kit
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Caspase 3 Activity Assay Protocol
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Caspase 3 Activity Assay
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Caspase 3 activity was determined using a Caspase Activity Assay Kit (Sigma) according to the manufacturer’s instructions.
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