Quercetin, isorhamnetin, miquelianin, B[a]P, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), nuclease P1, dimethyl sulfoxide (DMSO), and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Minimum essential medium (MEM), penicillin/streptomycin, fetal bovine serum (FBS), sodium pyruvate, and trypsin-EDTA were purchased from Welgene (Daegu, South Korea). Phosphate-buffered saline (PBS) was purchased from Biosesang (Seongnam, South Korea). Fluorescent mounting medium was purchased from Dako (Santa Clara, CA, USA). Antibodies (anti-NRF2, AhR, CYP1A1, GSTA1, CYP1A1, CYP1B1, ABCC2, ABCC3, and β-actin) and Alexa 488-conjugated anti-rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
Alexa 488 conjugated anti rabbit secondary antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Alexa 488-conjugated anti-rabbit secondary antibodies are fluorescently labeled secondary antibodies used for the detection of rabbit primary antibodies in immunoassays and imaging applications. The Alexa Fluor 488 dye attached to the secondary antibody emits green fluorescence upon excitation, allowing for the visualization and localization of target proteins or cellular structures recognized by the rabbit primary antibody.
Automatically generated - may contain errors
Lab products found in correlation
Miquelianin, by Merck Group (1 mentions)
Fluorescent mounting medium, by Agilent Technologies (1 mentions)
Phosphate buffered saline pbs, by Biosesang (1 mentions)
Anti nrf2, by Cell Signaling Technology (1 mentions)
Minimum essential medium (mem), by Welgene (1 mentions)
Abcc2, by Cell Signaling Technology (1 mentions)
Cyp1a1, by Cell Signaling Technology (1 mentions)
Abcc3, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Quercetin, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Isorhamnetin, by Merck Group (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Trypsin edta, by Welgene (1 mentions)
Cxcr6, by GeneTex (1 mentions)
Confocal microscopy, by Nikon (1 mentions)
2 protocols using alexa 488 conjugated anti rabbit secondary antibodies
1
Molecular Mechanisms of Phytochemical-Mediated AhR Modulation
2
Immunofluorescence Analysis of CXCR6 and CXCL16
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed with 4% paraformaldehyde to detect the cell membrane protein and blocked with 1% BSA for 30 min. Cells were then incubated with CXCR6 (GeneTex, USA) or CXCL16 (GeneTex) primary antibodies at 4°C overnight. To detect cells stained by primary antibodies, the cells were incubated with Alexa 488-conjugated anti-rabbit secondary antibodies (Cell Signaling, USA) for 45 min. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). Fluorescent images were obtained by confocal microscopy (Nikon) and quantitatively analyzed by the ImageJ program (National Institutes of Health, USA).
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