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2 protocols using erythropoietin

1

SARS-CoV-2 Spike Protein Detection

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Phosphate buffered saline (PBS), glycine, hydrochloric acid and tyrosine were purchased from Sigma Aldrich (USA). Tris(2-carboxyethyl) phosphine (TCEP) and 3-mercaptopropionic acid (MPA) were purchased from Thermo Scientific (USA). Recombinant spike S1 protein of SARS-CoV-2 (molecular weight = 78.3 KDa) and anti S1 monoclonal antibody were purchased from Sino Biological Inc. (China). Erythropoietin, troponin and insulin were purchased from Abcam (Australia). All preparations were made using deionized water (ultrapure Millipore, 18.2 MΩ cm, 25 °C). Gold coated silicon nanopillar substrate (Au@SNP) was outsourced from Silmeco ApS (Denmark), molecular sieve columns (illustra NAP-5) were outsourced from GE Healthcare Life Science (Australia).
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2

Comprehensive Metabolic Profiling in VSG

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Insulin (Crystal Chem), total GLP-1 (MesoScale Discovery), and acetaminophen (Sekisui Diagnostics) were measured during experiments shown in Fig. 3. Insufficient amount of blood was obtained from one Control VSG for Insulin, total GLP-1 and acetaminophen levels at 15 min. Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems), Myostatin (GDF8) (R&D Systems), FGF21 (R&D Systems), L-amino acids (Abcam), Erythropoietin (Abcam), FGF23 (Abcam), Ferritin (Abcam), Hemoglobin (Abcam), Phosphate (Abcam), 25(OH) Vitamin D (Abcam). Iron levels in cecal contents were measured with Iron Assay Kit (Abcam). Glycogen content was measured in liver and muscle (tibialis anterior) samples with Glycogen Assay kit (Sigma-Aldrich). All sampled blood was collected via tail vein in EDTA-coated tubes. All assays were performed according to the manufacturer’s instructions.
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