Cells on coverslips were fixed with 4% paraformaldehyde for 30 min, permeabilized with 0.1% Triton X-100, and blocked with 5% bovine serum albumin for 1 hour, followed by incubation with LC3 antibody (#4108, 1:500; Cell Signaling Technology, Danvers, MA, USA) at 4°C overnight. After washing with phosphate-buffered saline, cells were stained with FITC-labeled goat anti-rabbit IgG (ab6717, 1:1,000; Abcam, Cambridge, MA, USA) at 37°C for 1 hour. Cells were observed utilizing a confocal microscopy (Olympus, Tokyo, Japan).
Fitc labeled goat anti rabbit igg ab6717
Manufactured by Abcam
Sourced in United States
FITC-labeled goat anti-rabbit IgG (ab6717) is a secondary antibody conjugated with fluorescein isothiocyanate (FITC). It is designed to detect and bind to rabbit immunoglobulin G (IgG) in various immunoassays and research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc labeled goat anti rabbit igg ab6717
1
LC3 Immunofluorescence Staining Protocol
2
Immunofluorescence Antibody Staining
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Anti-Frizzled 7 (ab64636), anti-β-catenin (ab32572), and FITC-labeled goat anti-rabbit IgG (ab6717) were obtained from Abcam; anti-GAPDH (BP009232MO) were obtained from Cusabio; biotinylated lens culinaris agglutinin (LCA) (Z1010) and fluorescein labeled LCA (FL1041) were purchased from Vector; horseradish peroxidase (HRP)-labeled goat anti-mouse IgG (A0218), HRP-labeled goat anti-rabbit IgG (A0208) and HRP-labeled Streptavidin (A0303) were purchased from Beyotime. Andy Fluor 647 streptavidin secondary antibody (SA2K1469A) was purchased from GeneCopoeia.
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