Disruption beads

Manufactured by Research Products International

Disruption beads are spherical particles used in sample preparation for various analytical techniques. They are designed to facilitate the mechanical disruption and homogenization of biological samples, such as tissues, cells, or microorganisms, to aid in the extraction and recovery of target analytes.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (2 mentions) Speedvac, by Thermo Fisher Scientific (2 mentions) Optima lc ms grade water, by Thermo Fisher Scientific (2 mentions) Metabolomics amino acid mix standard, by Cambridge Isotopes (2 mentions) Beadblaster homogenizer, by Benchmark Scientific (2 mentions) Formic acid, by Merck Group (1 mentions)

Close spelling variants of this product

0.5-mm disruption beads (2 citations)

2 protocols using disruption beads

Metabolite Extraction and Sample Preparation

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Prior to extraction, samples were moved from −80°C storage to wet ice and thawed. Extraction buffer, consisting of 80% methanol (Cat# 615130025, Thermo Fisher) and 500 nM metabolomics amino acid mix standard (Cat# MSK-A2–1.2, Cambridge Isotope Laboratories), was prepared and placed on dry ice. Samples were extracted by mixing 50 μL of sample with 950 μL of extraction buffer in 2.0 mL screw cap vials containing ~100 μL of disruption beads (Cat# 9835, Research Products International). Each sample was homogenized for 10 cycles on a BeadBlaster homogenizer (Benchmark Scientific). Cycling consisted of a 30 s homogenization time at 6 m/s followed by a 30 s pause. Samples were subsequently spun at 21,000 rcf for 3 min at 4°C. A set volume of each (450 μL) was transferred to a 1.5 mL tube and dried down by SpeedVac (Thermo Fisher). Samples were reconstituted in 50 μL of Optima LC/MS grade water (Cat# W6500, Fisher Scientific). Samples were sonicated for 2 min, then spun at 21,000 rcf for 3 min at 4°C. Twenty μL were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed in −80°C for long term storage.

Ravn-Boess N., Roy N., Hattori T., Bready D., Donaldson H., Lawson C., Lapierre C., Korman A., Rodrick T., Liu E., Frenster J.D., Stephan G., Wilcox J., Corrado A.D., Cai J., Ronnen R., Wang S., Haddock S., Ortiz J.S., Mishkit O., Khodadadi-Jamayran A., Tsirigos A., Fenyö D., Zagzag D., Drube J., Hoffmann C., Perna F., Jones D.R., Possemato R., Koide A., Koide S., Park C.Y, & Placantonakis D.G. (2023). The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability. Cell reports, 42(11), 113374.

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Metabolomics Extraction and Sample Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols

Extraction buffer, consisting of 82% methanol (Fisher Scientific), 1% Formic Acid (Millipore Sigma) and 512 nM metabolomics amino acid mix standard (Cambridge Isotope Laboratories, Inc.), was prepared and placed on dry ice. Samples were extracted by mixing 25 μL of sample with 975 μL of extraction buffer in 2 mL screw cap vials containing ~100 μL of disruption beads (Research Products International, Mount Prospect, IL). Each sample was homogenized for 10 cycles on a bead blaster homogenizer (Benchmark Scientific). Cycling consisted of a 30 sec homogenization time at 6 m/s followed by a 30 sec pause. Samples were subsequently spun at 21,000 g for 3 min at 4 °C. A set volume of each (450 μL) was transferred to a 1.5 mL tube and dried down by speedvac (Thermo Fisher). Samples were reconstituted in 50 μL of Optima LC/MS grade water (Fisher Scientific). Samples were sonicated for 2 min, then centrifuged at 21,000 g for 3 min at 4 °C. 20 μL were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed in −80 °C for long term storage.

Zagury D., Lachgar A., Chams V., Fall L.S., Bernard J., Zagury J.F., Bizzini B., Gringeri A., Santagostino E., Rappaport J., Feldman M., O’Brien S.J., Burny A, & Gallo R.C. (1998). C-C chemokines, pivotal in protection against HIV type 1 infection. Proceedings of the National Academy of Sciences of the United States of America, 95(7), 3857-3861.

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