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Insulin assay kit

Manufactured by Mercodia
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The Insulin assay kit is a laboratory tool used to measure the concentration of insulin in biological samples. It provides a quantitative analysis of insulin levels, which is essential for various medical and research applications.

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Mouse insulin enzyme-linked immunosorbent assay (ELISA) kit Rat insulin enzyme-linked immunosorbent assay kit Insulin ELISA assay kit Ultra Sensitive Mouse Insulin enzyme-linked immunosorbent assay kit Ultrasensitive mouse insulin enzyme-linked immunosorbent assay (ELISA) kit High Range Rat Insulin ELISA assay kit Mouse insulin enzyme-linked immunosorbent assay kit Ultrasensitive mouse insulin assay kit Rat insulin assay kit Rat insulin Elisa assay kit

2 protocols using insulin assay kit

1

Plasma Lipid and Glucose Profiling

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Plasma total cholesterol and triglycerides were measured using kits from Raichem. Blood glucose was measured using the Accu-chek Aviva glucometer. Plasma insulin was measured using an insulin assay kit (Mercodia). The homeostasis model assessment of insulin resistance (HOMA-IR) was used as a measure of insulin resistance.
Adi N., Perriotte-Olson C., Desouza C., Ramalingam R, & Saraswathi V. (2015). Hematopoietic Cyclooxygenase-2 Deficiency Increases Adipose Tissue Inflammation and Adiposity in Obesity. Obesity (Silver Spring, Md.), 23(10), 2037-2045.
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2

Insulin Secretion in Glucose and IBMX

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Cells were preincubated for 30 min in Krebs Ringer bicarbonate buffer (Krebs and Henseleit, 1932) containing 10 mM HEPES pH 7.4, 2 mg/ml bovine serum albumin and 1 mM glucose, and then incubated for 1h in either 1 mM glucose, 20 mM glucose, 1 mM glucose + 0.5 mM IBMX or 20 mM glucose + 0.5 mM IBMX, at 37°C in Krebs Ringer Bicarbonate buffer with the same additions as during the pre-incubation.
Insulin concentrations were measured using an Insulin Assay Kit (catalog #: 10-1113-01, Mercodia).
Fred R.G., Kappe C., Ameur A., Cen J., Bergsten P., Ravassard P., Scharfmann R, & Welsh N. (2015). Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death. Molecular and cellular endocrinology, 414.
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