Control mirna

Manufactured by Thermo Fisher Scientific

Sourced in United States

Control miRNA is a synthetic RNA molecule that is designed to serve as a positive control for microRNA (miRNA) analysis. It is used to validate the performance of miRNA detection and quantification assays. Control miRNA provides a consistent and reliable reference point for monitoring the efficiency and accuracy of miRNA experiments.

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Lab products found in correlation

Close spelling variants of this product

Control miRNA mimic (6 citations) MirVana miRNA Mimic miR-1 Positive Control (4 citations) MirVana mimic miRNA control #1 (3 citations) Scrambled sequence miRNA control (2 citations) Control miRNA assay (2 citations) Cy-3-labelled control miRNA (2 citations) TaqMan endogenous control assay for miRNA-16 (2 citations) Pre-miR™ miRNA negative non-targeting control#1 (2 citations) TaqMan® Control miRNA Assay (2 citations) Pre-miR miRNA precursor control (2 citations)

26 protocols using control mirna

LNCOC1 Expression and Regulation

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Expression vector of LNCOC1 was constructed using pcDNA3.1 vector as backbone. miR-124 mimic, control miRNA, LNCOC1 knockdown negative control and LNCOC1 shRNA were synthesized by Sangon Biotech (Shanghai, China). Empty vector pcDNA3.1, control miRNA or LNCOC1 knockdown control RNA were used as negative control (NC). Following the manufacturer’s instructions of Lipofectamine 2000 (Lipo2000, Invitrogen), after SK-MEL-3 and A375 cells were harvested and counted, 10 nM expression vector, knockdown vector or 40 nM miRNA using were transfected with Lipo2000. miR-124 mimic, control miRNA, LNCOC1 shRNA and control shRNA were designed by Invitrogen, and the sequences were as following:
control miRNA: 5’-UUGUACUACACAAAAGUACUG-3’
miR-124 mimic: 5’-UAAGGCACGCGGUGAAUGCC-3’
LNCOC1 shRNA: 5’-AGTGCTCCTAGTGTTACCAGAG-3’
control shRNA: 5’-ACTAGCTAGGCATCGATATCAG-3’

Liu C., Ding X., Wei C., Pei Y., Meng F., Zhong Y, & Liu Y. (2022). LncRNA LNCOC1 is Upregulated in Melanoma and Serves as a Potential Regulatory Target of miR-124 to Suppress Cancer Cell Invasion and Migration. Clinical, Cosmetic and Investigational Dermatology, 15, 751-762.

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Chondrosarcoma Protein Signaling Pathway

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Rabbit monoclonal antibodies specific for p85, p-p85, Akt, p-Akt, FAK, and FAK as well as anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit monoclonal antibodies specific for VEGF-C and control IgG were purchased from Abcam (Cambridge, MA, USA). The ELISA kit for VEGF-C was obtained from PerpoTech (Rocky Hill, NJ, USA). The recombinant human VEGF-C was obtained from R&D Systems (Minneapolis, MN, USA). The human chondrosarcoma tissue array was obtained from Biomax (Rockville, MD, USA). The Matrigel was purchased from BD Biosciences (Bedford, MA, USA). The OBRl antisense and missense oligonucleotide (ODN) were purchased from MDBio (Taipei, Taiwan)31 (link). The Trizol, Lipofectamine 2000, MMLV RT kit, miR-27b mimic, miR-27b inhibitor and control miRNA were obtained from Invitrogen (Carlsbad, CA, USA). The siRNAs against p85, Akt1, FAK, and control were obtained from Dharmacon Research (Lafayette, CO, USA). The TaqMan assay kit and TaqMan MicroRNA Reverse Transcription Kit were obtained from Thermo Fisher Scientific (Grand Island, NY, USA). LY294002 and other pharmacological inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA)

Yang W.H., Chang A.C., Wang S.W., Wang S.J., Chang Y.S., Chang T.M., Hsu S.K., Fong Y.C, & Tang C.H. (2016). Leptin promotes VEGF-C production and induces lymphangiogenesis by suppressing miR-27b in human chondrosarcoma cells. Scientific Reports, 6, 28647.

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Signaling Pathway Modulation Assay

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Anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase as well as rabbit monoclonal antibodies (mAbs) specific for WISP-3, p38, p-p38 (Tyr¹⁸²), c-Src, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal antibody specific for p-Src (Tyr⁵²⁷) was purchased from Cell Signaling and Neuroscience (Danvers, MA, USA). A VEGF-A ELISA kit was obtained from PerpoTech (Rocky Hill, NJ, USA). Control IgG and rabbit mAb specific for VEGF-A were obtained from Abcam (Cambridge, MA, USA). Control miRNA, miR-452 mimic, MMLV RT kit, Trizol and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA, USA). The TaqMan MicroRNA Reverse Transcription kit and the TaqMan assay kit were obtained from Thermo Fisher Scientific (Grand Island, NY, USA). c-Src, p38 and control siRNA were purchased from Dharmacon Research (Lafayette, CO, USA). PP2 and SB203580 were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Lin C.Y., Tzeng H.E., Li T.M., Chen H.T., Lee Y., Yang Y.C., Wang S.W., Yang W.H, & Tang C.H. (2017). WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis. Oncotarget, 8(24), 39571-39581.

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miRNA Transfection and Analysis

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ACs and MG were transfected with the following miRNAs: hsa-miR-135-3p, hsa-miR-6790-3p, hsa-miR-11399, and control miRNA (Invitrogen, Carlsbad, CA, USA). Briefly, each 25 pmol miRNA was mixed with 7.5 μL Lipofectamine RNAiMAX (Invitrogen) in 100 μL Opti-MEM. The miRNA complexes were applied to 2 × 10⁵ cells. After 24 h, cells were collected and miRNA microarray analysis was performed.

Nishi K., Izumi H., Tomonaga T., Nagano C., Morimoto Y, & Horie S. (2023). IL-6-Mediated Upregulated miRNAs in Extracellular Vesicles Derived from Lund Human Mesencephalic (LUHMES) Cells: Effects on Astrocytes and Microglia. Biomolecules, 13(5), 718.

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In Vitro and In Vivo miRNA Modulation

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Control miRNA, control anti-miRNA, Pre-miR-Let7a (PM10050) and Anti–miR-Let7a (AM10050) for in vitro studies were from Applied Biosystems. MicroRNA was delivered using siPORT (Ambion). To evaluate microRNA expression levels, total RNA was extracted with Trizol (Invitrogen), and RT-PCR was performed to detect let-7a (Mm_let-7a-1_2), let 7d (Mm_let-7d_1), or let7f (Mm_let7f-1_1) miScript Primer Assay. Data were normalized to the internal control small RNA snoRNA202 (Applied Biosystems). For in vivo studies, oligomers were purchased from Sigma: Anti-miR scrambled control:5′[mG][mU][mC][mA][mA][mG][mG][mC][mA][mU]
[mC][mC][mG][mG][mA][mU][mC][mA][mU][mC][mA][mA]-3′
Anti-miR-Let7a:
5′[mA][mC][mU][mC][mC][mA][mU][mC][mA][mU][mC][mC][mA][mA][mC][mA][mU][mA][mU][mC][mA][mA]-3′

Schmid M.C., Khan S.Q., Kaneda M.M., Pathria P., Shepard R., Louis T.L., Anand S., Woo G., Leem C., Faridi M.H., Geraghty T., Rajagopalan A., Gupta S., Ahmed M., Vazquez-Padron R.I., Cheresh D.A., Gupta V, & Varner J.A. (2018). Integrin CD11b activation drives anti-tumor innate immunity. Nature Communications, 9, 5379.

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Colorectal Cancer Cell Line Transfection

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Colorectal cancer cell lines (HCT15, Colo205, LoVo, HT29, SW480, HCT116, DLD-1, SW620, SW48, LS513, and RKO) were purchased from American Type Culture Collection (ATCC) and cultured with RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented with 10% foetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) in a 37 °C incubator with 5% CO₂. For transient transfection, cells were transfected with indicated siRNA or miRNA using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. SiRNAs and miRNAs used in this study were as follows: control miRNA (4464058, Applied Biosystems), and vimentin siRNA (sc-29522, Santa Cruz Biotechnology, CA, USA), miR-17-5p mimic (4464066, Applied Biosystems), and miR-17-5p inhibitor (4464084, Applied Biosystems). All experiments were performed with mycoplasma-free cells.

Kim T.W., Lee Y.S., Yun N.H., Shin C.H., Hong H.K., Kim H.H, & Cho Y.B. (2020). MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer. British Journal of Cancer, 123(7), 1123-1130.

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miRNA-132 Transfection in Cells

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The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer’s protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer’s protocols for 24 h.

Connor Y., Tekleab S., Nandakumar S., Walls C., Tekleab Y., Husain A., Gadish O., Sabbisetti V., Kaushik S., Sehrawat S., Kulkarni A., Dvorak H., Zetter B., R. Edelman E, & Sengupta S. (2015). Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nature Communications, 6, 8671.

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TNBC Cell Line MDA-MB-231 Transfection

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TNBC cell line MDA-MB-231, purchased from American Type Culture Collection (ATCC), was used in the studies and was cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented by 10% FBS and 1% P/S. miRNAs, anti-miRNAs, control miRNA, and lipofectamin RNAiMAX were purchased from Life Technologies (Grand Island, NY).

Liu Y., Cai Q., Bao P.P., Su Y., Cai H., Wu J., Ye F., Guo X., Zheng W., Zheng Y, & Shu X.O. (2015). Tumor Tissue MicroRNA Expression in Association with Triple Negative Breast Cancer Outcomes. Breast cancer research and treatment, 152(1), 183-191.

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Synthetic Soya-Cerebroside Protocol

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Soya-cerebrosides (Fig. 1A) of >98% purity were synthesized at the College of Pharmacy, School of Pharmacy, China Medical University, Taichung, Taiwan (The detail synthesis procedures and purified quality was provided as described previously21 (link)). Soya-cerebroside was dissolved in DMSO for intraperitoneal (IP) injection. A miR-432 inhibitor, Control miRNA and TRIzol were from Life Technologies (Carlsbad, CA). Rabbit polyclonal antibody specific for MCP-1, MCP-1 enzyme-linked immunosorbent assay (ELISA) kit and human recombinant IL-1β were purchased from R&D Systems (Minneapolis, MN, USA), anti-phospho AMPK (Thr172) and phospho AKT (Ser473) from Cell Signaling (Danvers, MA, USA). Mouse monoclonal CD68 was purchased from Novus (Novus, Littleton, USA) and Ara A (ATP mimetic), Compound C (AMPK kinase inhibitor), and AKTi (AKT1/2 kinase inhibitor) from Enzo Biochem, Inc. (Enzo, New York, NY). Anti-AMPK, anti-AKT, and anti-mouse and anti-rabbit conjugated horseradish peroxidase (HRP) antibodies were from Santa Cruz (Santa Cruz, CA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Liu S.C., Chiu C.P., Tsai C.H., Hung C.Y., Li T.M., Wu Y.C, & Tang C.H. (2017). Soya-cerebroside, an extract of Cordyceps militaris, suppresses monocyte migration and prevents cartilage degradation in inflammatory animal models. Scientific Reports, 7, 43205.

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miRNA-132 Transfection Assay

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The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer's protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer's protocols for 24 h.

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