Ab125329

Manufactured by Merck Group

Ab125329 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory applications. The core function of this product is to [DESCRIPTION_NOT_AVAILABLE].

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Lab products found in correlation

Ripa cell lysis buffer, by Merck Group (2 mentions) Bis tris gel mops running buffer system, by Thermo Fisher Scientific (2 mentions) Rabbit anti rho gdi, by Merck Group (2 mentions) Horseradish peroxidase hrp conjugated donkey anti rabbit secondary antibody, by GE Healthcare (2 mentions)

2 protocols using ab125329

Western Blot Analysis of BMDM and Microglia

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Whole-cell extracts from BMDM and microglia per condition were prepared in ice-cold RIPA cell lysis buffer (Millipore, 20-188). Cell lysates were centrifuged at 13,000 rpm for 10 min at 4° C and equal amounts of protein were resolved with a 4 to 20 % Bis-tris Gel/MOPS running buffer system (Invitrogen) and transferred to nitrocellulose membranes. The membranes were analyzed by Western blotting with the following antibodies: rabbit anti-FENS1/WDFY1 (Abcam, ab125329), rabbit anti-Rho-GDI (Sigma, R3025), and horseradish peroxidase (HRP)-conjugated donkey anti-rabbit secondary antibody (GE Healthcare, NA934).

Babagana M., Oh K.S., Chakraborty S., Pacholewska A., Aqdas M, & Sung M.H. (2021). Hedgehog dysregulation contributes to tissue-specific inflammaging of resident macrophages. Aging (Albany NY), 13(15), 19207-19229.

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BMDM and Microglia Protein Extraction

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Whole-cell extracts from BMDM and microglia per condition were prepared in ice-cold RIPA cell lysis buffer (Millipore, 20-188). Cell lysates were centrifuged at 13,000 rpm for 10 min at 4 °C and equal amounts of protein were resolved with a 4 to 20 % Bis-tris Gel/MOPS running buffer system (Invitrogen) and transferred to nitrocellulose membranes. The membranes were analyzed by Western blotting with the following antibodies: rabbit anti-FENS1/WDFY1 (Abcam, ab125329), rabbit anti-Rho-GDI (Sigma, R3025), and horseradish peroxidase (HRP)-conjugated donkey anti-rabbit secondary antibody (GE Healthcare, NA934).

Babagana M., Oh K., Chakraborty S., Pacholewska A., Aqdas M., & Sung M. (2020). Hedgehog dysregulation contributes to tissue-specific inflammaging of resident macrophages.

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