Ac240ts cantilevers

Manufactured by Olympus

Sourced in United States

The AC240TS cantilevers are a type of lab equipment used in atomic force microscopy (AFM) applications. They are designed to measure the topography and properties of surfaces at the nanoscale level. The cantilevers are made of silicon and have a resonant frequency range of 50-90 kHz, suitable for a variety of sample types and scanning modes.

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Lab products found in correlation

Milli q water, by Merck Group (1 mentions) Mica surface, by Agar Scientific (1 mentions) Cypher s afm, by Oxford Instruments (1 mentions) Mfp 3d bio, by Oxford Instruments (1 mentions) Bx51 optical microscope, by Olympus (1 mentions) Cm12 transmission electron microscope, by Philips (1 mentions) Phosphate buffered saline pbs ph 7.4, by Thermo Fisher Scientific (1 mentions) Asylum research mfp 3d atomic force microscope, by Oxford Instruments (1 mentions) 1 undecanethiol, by Merck Group (1 mentions) Cypher es, by Oxford Instruments (1 mentions)

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AC240TS (19 citations) Cantilevers (8 citations)

6 protocols using ac240ts cantilevers

Atomic Force Microscopy of HET-s Prion Fibrils

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HET-s (218–289) prion fibrils grown in 20 mM citric acid (pH adjusted to 2.0 with HCl) were separated from supernatant by centrifugation and redispersed in pH 2.0 citric acid solution. The redispersed fibril solution was diluted 200X with pH 2.0 citric acid solution, whereas pH 6.0 HET-s (218–289) prion fibril solution was diluted 200X with distilled water (pH ~6.0). Then the fibrils were deposited on freshly cleaved mica; the surface was gently washed with pH 2.0 citric acid solution or distilled water, respectively and dried under nitrogen flow. AFM imaging was performed using a MFP3D™ Bio Asylum Research Microscope (Asylum Research, CA, USA) in AC mode with Olympus AC240TS cantilevers.
Igor Pro 6.2.1.0 software was used to prepare images. For the population density distribution analysis, the width of 50 different fibrils or filaments were measured using Igor Pro 6.2.1.0 software and plotted together using the Kernel Density Estimate¹⁰ program. One width measurement was made per fibril and the probability distribution plots (Figure 1, c) were normalized such that area under the curve is the same for both curves.

Shanmugasundaram M., Kurouski D., Wan W., Stubbs G., Dukor R.K., Nafie L.A, & Lednev I.K. (2015). Rapid Filament Supramolecular Chirality Reversal of HET-s (218–289) Prion Fibrils Driven by pH Elevation. The journal of physical chemistry. B, 119(27), 8521-8525.

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Imaging DNA Origami Porin on Mica

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Five μL of the DNA origami
porin (10 nM) in 10 mM Tris-HCl, 1 mM EDTA, 20 mM MgCl₂, pH 8.0 was deposited on a freshly cleaved mica surface (Agar Scientific)
and incubated for 90 s. Subsequently, the surface was rinsed 3×
with 1 mL of Milli-Q water (Merck Millipore) to remove excess sample
and blow-dried with nitrogen. Imaging was carried out using a Cypher
S AFM (Oxford Instruments) in amplitude modulation in air and at room
temperature using AC240TS cantilevers (Olympus) with a nominal spring
constant of 2 N/m. The set-point to free amplitude ratio was generally
kept around 70% with a free oscillation amplitude of 20 nm. The frequency
of excitation was set close to the resonance of the first flexural
mode (around 70 kHz), and a repulsive mode was preferred. The scan
speed was set to either 1 or 2 Hz obtaining an image of 256 ×
256 pixels. The images were flattened and band-pass filtered using
Gwyddion (http://gwyddion.net/). Image analysis was performed
as described in the Supporting Information, Note S1, Figures S6, S7, and Tables S7, S8.

Göpfrich K., Li C.Y., Ricci M., Bhamidimarri S.P., Yoo J., Gyenes B., Ohmann A., Winterhalter M., Aksimentiev A, & Keyser U.F. (2016). Large-Conductance Transmembrane Porin Made from DNA Origami. ACS Nano, 10(9), 8207-8214.

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AFM Imaging of Cells on Poly-L-Lysine

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Glass slides were modified using a poly‐l‐lysine fixation method to immobilize cells during AFM imaging.
²⁷ (link) Glass was cleaned with ethanol and immersed for 10 min in a solution of 0.05 mg/mL poly‐l‐lysine hydrobromide and 10 mM Tris (pH 8.0). The slides were covered, dried vertically overnight at room temperature, and used within a week. Approximately 100 µL of the dyed solutions were deposited onto modified glass slides, incubated for 30 min, and rinsed with 3 mL sterile H₂O. The microscope objective was calibrated using AFM software for precise image overlay on topographical AFM scans. Both optical and fluorescence images were taken in the same location and are overlaid using image analysis software. Non‐contact mode topographical scans of the same cells were taken with an AFM (MFP‐3D Bio, Asylum Research) using AC240TS cantilevers (Olympus), cleaned with high‐intensity UV light to remove organic contamination. The final overlay of the optical/fluorescence image and topographical AFM images was performed in IgorPro software and 3D images of fluorescence distribution could be created.

Olsen A., Ehrhardt C.J, & Yadavalli V.K. (2020). Nanoscale visualization of extracellular DNA on cell surfaces. Analytical Science Advances, 1(3), 194-202.

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Microscopic Characterization of Propionibacteria

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Propionibacteria were routinely examined as wet-mount fresh cultures using an immersion phase contrast × 100 objective on an Olympus BX51 optical microscope. As an alternative, cultures were dried on a mica slide prior to analysis using AFM (Atomic Force Microscopy, as previously described (Deutsch et al., 2012 (link)). Briefly, the bacteria were washed in HEPES-NaCl buffer, deposited onto a freshly cleaved disk of mica and allowed to dry for 24 h in a desiccator. AFM imaging was performed in air, at a controlled temperature of 20°C and using a MFP-3D-BIO microscope (Oxford Instruments, Asylum Research, Santa Barbara, CA, United States). Images were acquired in tapping mode using AC240TS cantilevers (Olympus, Tokyo, Japan). Transmission Electron Microscopy (TEM) was performed as described previously (Deutsch et al., 2010 (link)). Briefly, bacteria were washed in PBS, fixed using glutaraldehyde, postfixed using osmium tetroxide/potassium cyanoferrate/uranyl acetate and dehydrated in ethanol (30–100%) prior to embedding in Epon. Thin sections (70 nm) were collected on 200-mesh copper grids and counterstained with lead citrate before examination using a Philips CM12 transmission electron microscope.

Tarnaud F., Gaucher F., do Carmo F.L., Illikoud N., Jardin J., Briard-Bion V., Guyomarc’h F., Gagnaire V, & Jan G. (2020). Differential Adaptation of Propionibacterium freudenreichii CIRM-BIA129 to Cow’s Milk Versus Soymilk Environments Modulates Its Stress Tolerance and Proteome. Frontiers in Microbiology, 11, 549027.

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Functionalization of Mica Surfaces with Alkanethiols

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We purchased 1-undecanethiol from Sigma-Aldrich (St. Louis, MO, USA). 3 aminopropyltri-ethoxysilane (APTES), glutaraldehyde (25% aq. solution), Phosphate-buffered saline (PBS pH 7.4) (11.9 mM phosphates, 137 mM sodium chloride and 2.7 mM potassium chloride) and ethanol (200 proof or 100% ethanol) were purchased from Fisher Scientific (Waltham, MA USA). Mica was purchased from Ted Pella (Redding, CA, USA). AC240TS cantilevers (Olympus, Center Valley, PA, USA) were used for non-contact mode imaging in air, while gold coated TR800PB cantilevers (Olympus) were used for force measurements. To prevent contamination, cantilevers were cleaned using an UV/Ozone Procleaner (BioForce Nanosciences Inc. Ames, IA, USA) before use. Imaging and force spectroscopy experiments were performed on an Asylum Research MFP-3D atomic force microscope (Oxford Instruments, Santa Barbara, CA, USA).

Iqbal K.M., Bertino M.F., Shah M.R., Ehrhardt C.J, & Yadavalli V.K. (2020). Nanoscale Phenotypic Textures of Yersinia pestis Across Environmentally-Relevant Matrices. Microorganisms, 8(2), 160.

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AFM Characterization of Polymer Layer

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The thickness of the N 3 -PG 110 -b-P(Cat 5 -Ph 5 -A 2 ) polymer layer was determined by AFM scratching experiments on a Cypher ES (Asylum Research, an Oxford Instruments company, Santa Barbara, CA, USA) using AC240TS cantilevers (Olympus, Japan). AFM imaging of those polymer layers was performed in pH 6.0 MOPS buffer (0.1 M) at a temperature of 25 °C. The first image of each sample was taken in the intermittentcontact mode with a scan size of 15•15 μm 2 . Then, the surface was scratched in contact mode with a scan size of 1•1 μm 2 and an applied force (trigger force) of 648 nN. The imaging mode was switched back again to intermittent-contact mode for further imaging. All images were taken at a scan rate of 2 Hz. The depth of the hole, created by the cantilever tip in contact mode, was used to determine the presence and thickness of the polymer layer on the respective substrate.

Lallemang M., Yu L., Cai W., Rischka K., Hartwig A., Haag R., Hugel T, & Balzer B.N. (2022). Multivalent non-covalent interactions lead to strongest polymer adhesion. Nanoscale, 14(10).

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