Microscopy acquisitions were performed using a Nikon Ti2-E inverted microscope (Nikon, Japan) equipped with a ×20/0.45 NA S Plan Fluor objective lens (Nikon, Switzerland) and a Nikon DS-Qi2 monochrome microscope camera. Images and videos taken in the bright field channel were acquired using a Ti2 Illuminator-DIA and an exposure time of 20ms. GA1 cells strained with RedoxSenor Green (RSG) (BacLight vitality kit, Thermofisher) were visualized by conventional epifluorescence microscopy with a ×100/1 oil NA S Plan Fluor objective lens (Nikon, Switzerland). A lumencor sola illuminator (Lumencor, USA) was used as the source of excitation with an exposure time of 500ms and the GFP-B HC Bright-Line Basic Filter was used. Acquisitions were then processed with NIS-Element AR software (Nikon, Japan).
Ds qi2 monochrome microscope camera
Manufactured by Nikon
Sourced in Switzerland, Japan
The DS-Qi2 is a monochrome microscope camera from Nikon. It features a high-resolution sensor and is designed for capturing detailed images in microscopy applications.
Automatically generated - may contain errors
4 protocols using ds qi2 monochrome microscope camera
1
Microscopy Imaging of Cellular Redox State
2
Inducible cyfip2-EGFP Expression Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Inducible expression of cyfip2-EGFP, as well as C179R and K723E variants, was initiated at 30 hpf by placing dechorionated larvae into 96-well plates and incubating at 38°C for 15 or 40 minutes [32 (link)]. Following heatshock, larvae were returned to Petri dishes, and given 4 days of recovery at 29°C. GFP fluorescence was confirmed between 4–6 hours post-heatshock for startle experiments using a Nikon SMZ25 stereo microscope with a GFP bandpass filter and Lumen 200 fluorescence illumination system. For day 5 heatshock rescue experiments, larvae were given 4 hours of recovery at 29°C prior to startle sensitivity testing.
For imaging experiments, larvae were treated as above for transgene expression at 30 hpf and at 29°C for 1 hour recovered in petri dishes in groups ≤ 65. After 1 hour of recovery fluorescence was verified, and larvae were visualized using the stereo microscope system described above and larval images were captured at 1-, 3-, 6-, 18-, 24-, 30-, and 42-hours post-heatshock using a Nikon DS-Qi2 monochrome microscope camera. Image analysis was conducted using FIJI (ImageJ) analysis software to manually define ROIs encompassing the entire larval body, excluding the eye and auto fluorescent yolk sac. Fluorescence intensity values reflect the mean gray values recorded for respective ROIs.
For imaging experiments, larvae were treated as above for transgene expression at 30 hpf and at 29°C for 1 hour recovered in petri dishes in groups ≤ 65. After 1 hour of recovery fluorescence was verified, and larvae were visualized using the stereo microscope system described above and larval images were captured at 1-, 3-, 6-, 18-, 24-, 30-, and 42-hours post-heatshock using a Nikon DS-Qi2 monochrome microscope camera. Image analysis was conducted using FIJI (ImageJ) analysis software to manually define ROIs encompassing the entire larval body, excluding the eye and auto fluorescent yolk sac. Fluorescence intensity values reflect the mean gray values recorded for respective ROIs.
3
Imaging Biofilm Pellicle Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pictures of biofilm pellicles of GA1 formed at the air-liquid interface were taken with a Nikon SMZ1270 stereomicroscope (Nikon, Japan) equipped with a Nikon DS-Qi2 monochrome microscope camera and a DS-F 1× F-mount adapter. Pictures were captured in the bright field channel with an ED Plan Apo 1×/WF objective and a 0.63x magnification of with an exposure time of 40ms. Additionally, pictures of biofilm pellicles at 6h were captured with a 3x magnification and an exposure time of 10ms to get a better view of the structures in formation. Acquisitions were then processed with NIS-Element AR software (Nikon, Japan).
4
Amyloid Beta Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neuron Cellular Viability in Presence of Amyloid Beta. Aβ 1-42 was prepared as previously described. The cells were incubated for 6, 24, 36, and 48 hours. PI staining was applied through addition of 10 μL of PI 45 minutes prior to fixation. The cells were fixed with 500 μL of 4% formaldehyde followed by 400 μL of 2% formaldehyde. The fixing agent was removed with 3 consecutive PBS (1x) washes.
Microscopy. Fine tweezers were used to plate the cells fixed on 12 mm round cover slips. The cells were counter-stained with DAPI for nuclear visualization and imaged using a Nikon Eclipse Ni-E with fluorescent filters and a DS-Qi2 monochrome microscope camera. 20x magnification was used with appropriate filters for DAPI and PI stained cell images. Five images were acquired for each cell culture in order to obtain the average cell deaths vs. total cells per culture. ImageJ software was used to process and analyze the cell images obtained.
Microscopy. Fine tweezers were used to plate the cells fixed on 12 mm round cover slips. The cells were counter-stained with DAPI for nuclear visualization and imaged using a Nikon Eclipse Ni-E with fluorescent filters and a DS-Qi2 monochrome microscope camera. 20x magnification was used with appropriate filters for DAPI and PI stained cell images. Five images were acquired for each cell culture in order to obtain the average cell deaths vs. total cells per culture. ImageJ software was used to process and analyze the cell images obtained.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!