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Cd27 bv605 clone l128

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CD27-BV605 (clone L128) is a fluorochrome-conjugated monoclonal antibody that binds to the CD27 antigen. CD27 is a member of the tumor necrosis factor receptor superfamily and is expressed on various lymphocyte subsets. This product is intended for use in flow cytometry applications to identify and study CD27-positive cells.

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Lab products found in correlation

Fixable viability stain 510, by Thermo Fisher Scientific (2 mentions) Cd45ro bv421 clone uchl1, by BD (2 mentions) P24 kc57 fitc, by Beckman Coulter (2 mentions) Biocoll cell separation solution, by Harvard Bioscience (1 mentions) Facslyric, by BD (1 mentions) Cd19 pc7 clone j3 119, by Beckman Coulter (1 mentions) Cd3 af700 clone ucht1, by BD (1 mentions) Cd4 pe cy7 clone l200, by BD (1 mentions) Cd25 bv421 clone m a251, by BD (1 mentions) Cd4 bv786 clone sk3, by BD (1 mentions) Cd69 apc, by BioLegend (1 mentions) Cd69 percp cy5 5 clone fn50, by BD (1 mentions)

Spelling variants of this product

CD27 BV605 (2 citations)

3 protocols using cd27 bv605 clone l128

1

PBMC Isolation and Flow Cytometry

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Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by density gradient centrifugation with Biocoll cell separation solution (Biochrom AG, Berlin, Germany) and frozen in FCS + 10% DMSO. For analysis, PBMCs were thawed and washed twice with PBS. One million cells were stained with smCD3-APC-H7 (clone SK7, BD), CD19-PC7 (clone J3-119, Beckman Coulter), CD20-V450 (clone 2H7, BioLegend), CD27-BV605 (clone L128, BD) and CD38-APC-R700 (clone HIT2, BD for 30 min at room temperature. Flow cytometry was performed on a BD FACSLyricTM and data was analyzed using FlowJo software (version 10).
Schultheiß C., Paschold L., Willscher E., Simnica D., Wöstemeier A., Muscate F., Wass M., Eisenmann S., Dutzmann J., Keyßer G., Gagliani N, & Binder M. (2021). Maturation trajectories and transcriptional landscape of plasmablasts and autoreactive B cells in COVID-19. iScience, 24(11), 103325.
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2

Multicolor Flow Cytometry Analysis

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Fixable Viability Stain 510 was obtained from ThermoFisher Scientific (#L34957; 1/1000 dilution). The following antibodies were used in sorting experiments: CD8-AF700 Clone RPA-T8 (ThermoFisher, #56-0088-41; 1/200 dilution), CD45RO-BV421 Clone UCHL1 (BD Biosciences, #562649; 1/100 dilution), CD27-BV605 Clone L128 (BD Biosciences, #562656; 1/100 dilution). For p24 staining, we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, #6604665; 1/500 dilution) and p24 28B7-APC (MediMabs, #MM-0289-APC; 1/400 dilution).
Cole B., Lambrechts L., Gantner P., Noppe Y., Bonine N., Witkowski W., Chen L., Palmer S., Mullins J.I., Chomont N., Pardons M, & Vandekerckhove L. (2021). In-depth single-cell analysis of translation-competent HIV-1 reservoirs identifies cellular sources of plasma viremia. Nature Communications, 12, 3727.
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3

Multiparameter Flow Cytometry Panel

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Fixable Viability Stain 510 was obtained from ThermoFisher Scientific (#L34957, 1/1000). The following antibodies were used in staining experiments: CD3 AF700 Clone UCHT1 (BD Biosciences, #557943, 1/50), CD4 PE-Cy7 Clone L200 (BD Bioscience, #560644, 1/200) and CD4 BV786 Clone SK3 (BD Biosciences, #563881, 1/200), CD8 BV510 Clone RPA-T8 (BioLegend, #301047, 1/200), CD45RO BV421 Clone UCHL1 (BD Biosciences, #562649, 1/100) or CD45RO PE Clone HI100 (BD Biosciences, #5555493, 1/20), CD27 BV605 Clone L128 (BD Biosciences, #562656, 1/100), PD1 BB700 Clone EH12.1 (BD Biosciences, #566461, 1/100), CD69 PerCP-Cy5.5 Clone FN50 (BD Biosciences, #560738) and CD69 APC (BioLegend, #310909, 1/50), CD25 BV421 Clone M-A251 (BD Biosciences, #562443, 1/50), HLA-DR FITC Clone REA805 (Miltenyi Biotech, #130-111-788, 1/50), GZMA PE-Cy7 Clone CB9 (BioLegend, #507221, 1/25), GZMB PB Clone GB11 (BioLegend, #515407, 1/50), CCL5 PerCP-Cy5.5 Clone VL1 (BioLegend, #515507, 1/50), CD127 PE-CF594 Clone HIL-7R-M21 (BD Biosciences, #562397, 1/50). For p24 staining, we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, #6604665, 1/500) and p24 28B7-APC (MediMabs, #MM-0289-APC, 1/400).
Pardons M., Cole B., Lambrechts L., van Snippenberg W., Rutsaert S., Noppe Y., De Langhe N., Dhondt A., Vega J., Eyassu F., Nijs E., Van Gulck E., Boden D, & Vandekerckhove L. (2023). Potent latency reversal by Tat RNA-containing nanoparticle enables multi-omic analysis of the HIV-1 reservoir. Nature Communications, 14, 8397.
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