Asparagine (99%) was purchased from Sigma-Aldrich, carbohydrates (>98%) (glucose, fructose, sucrose) from Fluka, acrylamide (>99%) from Merck (Darmstadt, Germany), 2,3,3-d3-acrylamide (>98%) from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), from Sigma- Aldrich (St. Louis, MO, USA), ethyl acetate and methanol (both of HPLC-grade) from J. T. Baker (Deventer, The Netherlands), and HPLC-grade acetonitrile from Scharlab (Barcelona, Spain). Hexane (99%), Br (99.5%), anhydrous sodium acetate (>99%), bromine water, formic acid, Carrez I, and Carrez II were from Chempur (Piekary Śląskie, Poland). Ultra-purified water (resistivity 18.2 MΩ cm−1) was obtained using Milli-Q Plus Technology, Millipore (Bedford, MA, USA). C 18-E (55μm, 70Å, 6 mL, 500 mg) SPE cartridges were purchased from Shim-Pol (Izabelin, Poland).
2 3 3 d3 acrylamide
Manufactured by Cambridge Isotopes
Sourced in United States
2,3,3-d3-acrylamide is a deuterated organic compound used as a labeling reagent in various analytical and research applications. It is a stable isotope-labeled variant of the common chemical acrylamide, with three deuterium atoms substituting the hydrogen atoms at specific positions in the molecule.
Automatically generated - may contain errors
Lab products found in correlation
Acrylamide, by Merck Group (2 mentions)
Asparagine, by Merck Group (1 mentions)
Hexane, by Chempur (1 mentions)
Formic acid, by Chempur (1 mentions)
Bromine water, by Chempur (1 mentions)
Sodium acetate anhydrous, by Chempur (1 mentions)
Carrez 1, by Chempur (1 mentions)
Carrez 2, by Chempur (1 mentions)
Hplc grade acetonitrile, by Scharlab (1 mentions)
Ethyl acetate, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Analytical grade methanol, by Merck Group (1 mentions)
Ferrous gluconate, by Merck Group (1 mentions)
Elix milli q water purification system, by Merck Group (1 mentions)
Acetic acid solution, by ITW Reagents (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
3 protocols using 2 3 3 d3 acrylamide
1
Analytical Procedure for Asparagine and Carbohydrates
2
Asparagine and Saccharide Analysis Protocol
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The reference standard of asparagine was purchased from Sigma-Aldrich. The standards of saccharides (glucose, fructose, maltose, ribose, and sucrose) were supplied by Fluka. Acrylamide (99%) was procured from Merck, and 2,3,3-d3-Acrylamide (>98%) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Acetonitrile (99.9%) was purchased from Sigma-Aldrich. All other reagents were of analytical grade and supplied by POCH (Gliwice, Poland).
3
Analytical Reagent Procurement for Research
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Acetic acid solution was purchased from PANREAC APPLICHEM® (Darmstadt, Germany). Ferrous gluconate and sodium chloride were supplied by Sigma-Aldrich (St. Louis, MO). Calcium chloride was obtained by Tetra Chemicals (Helsingborg, Sweden). Acrylamide (>99 %) was purchased from Fluka (Buchs SG, Switzerland). 2,3,3-D 3 -acrylamide (98 %) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). Analytical grade methanol was supplied by Merck (Darmstadt, Germany). A nylon syringe filter was obtained from the). FILTER-LAB (Barcelona, Spain). ISOLUTE Multimode (300 mg, 6 mL) and ISOLUTE ENV+ (200 mg, 3 mL) solid phase extraction cartridges were obtained from International Sorbent Technology (Hengoed, Mid Glamorgan, UK). Nylon and nitrocellulose syringe filters (0.45 μm) were purchased from Tracer Analytical Technologies (Madrid, Spain). Water was purified with an Elix/Milli-Q water purification system (Millipore, Bedford, MA, USA).
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