Saliva samples were collected from infants using assisted-collection sponges and kits (CS-2 sponges and OG-250 kits) and from mothers using passive drool collection tubes (OG-500 kit) from DNA Genotek, Ottawa, Canada. Samples were stored at room temperature until DNA isolation. DNA was isolated using the manual purification protocol from DNA Genotek. Genotyping of CD38 rs3796863 was performed with a 5ʹ-nuclease assay using primers and probes from Applied Biosystems (TaqMan® SNP Genotyping Assay). Polymerase Chain Reaction was conducted with HotStarTaq Plus DNA Polymerase and Q-solution (Qiagen, Venlo, Netherlands) in a BioRad C1000 thermocycler with a CFX96 fluorescence reading module, using the following thermal protocol: denaturation at 95°C for 5 min; followed by cycling: 95°C for 15 s and 60°C for 1 min for 45 cycles.
Og 250 kits
Manufactured by DNA Genotek
Sourced in Canada
The OG-250 kits are a collection of laboratory equipment and supplies designed for DNA collection and stabilization. The kits include a saliva collection device, a stabilization liquid, and other necessary components to facilitate the collection and preservation of DNA samples. The core function of the OG-250 kits is to enable the safe and reliable collection of DNA samples for various analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Cs 2 sponges, by DNA Genotek (2 mentions)
C1000 thermocycler, by Bio-Rad (1 mentions)
Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions)
Genotyping module v1, by Illumina (1 mentions)
Genomestudio software v2011, by Illumina (1 mentions)
Prepit l2preagent, by DNA Genotek (1 mentions)
Hydration solution, by Qiagen (1 mentions)
3 protocols using og 250 kits
1
Infant and Maternal Saliva DNA Genotyping
2
APOE Genotyping and Ancestry Analysis
3
Infant Saliva DNA Collection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Passive drool was collected from infants at 5 months of age using CS-2 sponges and OG-250 kits (DNA Genotek, Ottawa, Canada) and was stored at room temperature until DNA isolation. We incubated collection kits at 50 °C for 1 h, then centrifuged for 10 min at 200 rcf to release all liquid from sponges. We isolated DNA from 500 μL of saliva using the manual purification protocol from DNA Genotek. DNA was stored in Hydration Solution (10 mM Tris, 1 mM EDTA, pH 7–8, Qiagen, Valencia, CA) and quantitated using nanodrop. One infant was excluded from the final analysis due to insufficient DNA.
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