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Adi spa 223

Manufactured by GeneTex
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The ADI-SPA-223 is a piece of laboratory equipment designed for sample preparation. It provides a compact and efficient platform for automated solid-phase extraction (SPE) processing. The device can handle a variety of sample types and is suitable for a range of analytical applications.

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Lab products found in correlation

D9564, by Merck Group (2 mentions) Prb 278p, by Merck Group (2 mentions) Prb 435p, by Merck Group (2 mentions) Z0334, by Novus Biologicals (2 mentions) Ab15766, by GeneTex (2 mentions) Gtx62440, by Merck Group (2 mentions) Gtx11268, by Fortrea (2 mentions) Mab377, by Fortrea (2 mentions)

2 protocols using adi spa 223

1

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
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2

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
+ Open protocol
+ Expand

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