The reagents used in this study were purchased from the following suppliers: Dulbecco's modified Eagle's medium/Ham's F12 nutrient mixture (DMEM/F12) and selenium from Gibco BRL (Gaithersburg, MD, USA); fetal bovine serum (FBS), trypsin-EDTA, and penicillin/streptomycin from Cambrex Bio Technology (Walkersville, MD, USA); 3-[4,5-dimetjylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), anti-β-actin, RIA-grade bovine serum albumin (BSA), and transferrin from Sigma-Aldrich Co. (St. Louis, MO, USA); antibodies against cyclin D1 and phospho-Rb (Ser807/811) from Cell Signaling Technology (Beverly, MA, USA); antibodies against p21CIP1/WAF1 (c-19), p27KIP1, p53, CDK2 (M-2), CDK4 (c-22), E2F-1 (C-20), and Rb (c-15) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Unless otherwise noted, all other materials were obtained from Sigma-Aldrich Co.
Rb c 15
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rb (c-15) is a primary antibody that targets the Rb (c-15) epitope. It is commonly used in immunochemical techniques such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Selenium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium ham s f 12 nutrient mixture dmem f12, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Transferrin, by Merck Group (1 mentions)
Phospho rb ser807 811, by Cell Signaling Technology (1 mentions)
Flag epitope, by Merck Group (1 mentions)
Phospho creb ser133, by Cell Signaling Technology (1 mentions)
Nanog d73g4, by Cell Signaling Technology (1 mentions)
Sox2 d6d9, by Cell Signaling Technology (1 mentions)
Cyclin a c 19, by Santa Cruz Biotechnology (1 mentions)
N cadherin h 63, by Santa Cruz Biotechnology (1 mentions)
Gst b 14, by Santa Cruz Biotechnology (1 mentions)
Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions)
C myc, by Santa Cruz Biotechnology (1 mentions)
β actin, by Merck Group (1 mentions)
Pax6 prb 278p, by Fortrea (1 mentions)
Vimentin, by Abcam (1 mentions)
Cyclin d1, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rb c 15
1
Cell Proliferation Assay Reagents
2
Antibody Characterization for Stem Cell Markers
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The antibodies used were: policlonal VRK1 (VC) and monoclonal VRK1 (1B5 or 1F6)64 (link). VRK1 (HPA000660, Sigma). Sox2 (D6D9, Cell Signaling; Berverly, MA,; E4, Santa Cruz; Santa Cruz, CA; Y-17, Santa Cruz,). Oct4 (2840, Cell Signaling), Nanog (D73G4, Cell Signaling), N-Cadherin (H-63, Santa Cruz), Vimentin (RV202, Abcam; Cambridge, UK). Flag epitope (Sigma-Aldrich, monoclonal M5; Sigma-Aldrich, polyclonal F7425). HA epitope (Santa Cruz, F-7; Sigma-Aldrich, H6908), myc epitope (06–549, Millipore; Billerica, MA; 05–724, Millipore), GST (B-14, Santa Cruz). Cyclin D1 (M-20, Santa Cruz). Cyclin A (C-19, Santa Cruz). Rb (C-15, Santa Cruz). Phospho-Rb (Ser807/811)(9308, Cell Signaling). PCNA (PC10, Santa Cruz). PAX6 (PRB-278P, Covance; Princeton, NJ), p27 (610241, BD-Transduction Laboratories; Franklin Lakes, NJ). CREB (9104, Cell Signaling). Phospho-CREB (Ser133) (9191, Cell Signaling). C-myc (N-262, Santa Cruz). β-actin (AC-15, Sigma-Aldrich). Secondary antibodies goat α-Mouse IgG, DyLightTM 680 and/or goat α-Rabbit Ig-G, DyLightTM 800 (Thermo Fischer Scientific) were used for detection in a Li-Cor Odyssey system (Thermo Fisher Scientific; Waltham, MA).
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