Methylcellulose medium

Manufactured by R&D Systems

Sourced in United States

Methylcellulose medium is a viscous liquid solution used as a cell culture medium component. It helps maintain the suspension of cells in culture by increasing the viscosity of the medium.

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Lab products found in correlation

Evos xl core microscope, by Thermo Fisher Scientific (1 mentions) Neomycin, by Thermo Fisher Scientific (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Human methylcellulose complete media, by R&D Systems (1 mentions)

Spelling variants of this product

Methylcellulose (51 citations) Human methylcellulose base medium (11 citations) Methylcellulose base medium (3 citations) Human methylcellulose-based medium (2 citations) HSC003 methylcellulose medium (2 citations)

3 protocols using methylcellulose medium

INA6 Myeloma Colony Formation Assay

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Briefly, INA6 myeloma cells (2 × 10³ cells/ml) were suspended in methylcellulose medium (R&D systems) supplemented with 10% serum from myeloma patients, 50 μg/ml penicillin or 50 μg/ml streptomycin or 100 μg/ml Neomycin (Gibco, Grand Island, NY, USA) and 20 ng recombinant human IL-6 as a growth factor for INA6 MM cells. At the end of the 2 weeks, INA6 colonies (>30 cells) were counted under Evos XL core microscope (Life Technologies, Grand Island, NY, USA).

Bam R., Venkateshaiah S.U., Khan S., Ling W., Randal S.S., Li X., Zhang Q., van Rhee F., Barlogie B., Epstein J, & Yaccoby S. (2014). Role of Bruton's tyrosine kinase (BTK) in growth and metastasis of INA6 myeloma cells. Blood Cancer Journal, 4(8), e234-.

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Leukemia Cell Line Culture and Assays

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The human leukemia cell lines MV4-11, K562 and THP1 were obtained from ATCC and maintained in Roswell Park Memorial Institute 1640 medium (RPMI-1640) supplemented with 10% fetal bovine serum (FBS), 100 IU/ml penicillin and 100 μg/ml streptomycin. The 293FT cells were maintained in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% FBS, 100 IU/ml penicillin, 100 μg/ml streptomycin and MEM Nonessential Amino Acids Solution. For the colony forming assays, the human leukemia cells were transduced with lentiviral shRNA and plated in triplicate in either 1.2% methylcellulose medium (R&D systems) supplemented with 10% FBS (for MV4-11 and K562) or Human Methylcellulose Complete Media (R&D systems) containing human EPO, GM-CSF, IL-3 and SCF (for THP1).

Hattori A., McSkimming D., Kannan N, & Ito T. (2017). RNA binding protein MSI2 positively regulates FLT3 expression in myeloid leukemia. Leukemia research, 54, 47-54.

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Quantifying Colony Formation in HSCs

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Control CD34+ HSC and primary AML cells (1×10³ to 5×10⁴ cells) were plated in methylcellulose medium (R&D systems) and colonies were visualised, measured and counted after 10 days. For normal CD34+ CFU-GM, CFU-M, CFU-M and BFU-E was measured.

Pillinger G., Loughran N.V., Piddock R.E., Shafat M.S., Zaitseva L., Abdul-Aziz A., Lawes M.J., Bowles K.M, & Rushworth S.A. (2016). Targeting PI3Kδ and PI3Kγ signalling disrupts human AML survival and bone marrow stromal cell mediated protection. Oncotarget, 7(26), 39784-39795.

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