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Anti ifn γ pe cyanine7 clone xmg1

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Anti-IFN-γ-PE/Cyanine7 (clone XMG1.2) is a fluorescent-conjugated antibody used in flow cytometry applications to detect and quantify interferon-gamma (IFN-γ) expression.

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Lab products found in correlation

Fixation buffer, by BioLegend (2 mentions) Intracellular staining perm wash buffer, by BioLegend (2 mentions) Anti il 4 pe clone 11b11, by BioLegend (2 mentions) Anti cd4 fitc clone gk1, by BioLegend (2 mentions) Anti 1 a 1 e percp clone m5 114, by BioLegend (2 mentions) Anti cd86 pe clone gl 1, by BioLegend (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (2 mentions) Macsquant analyzer, by Miltenyi Biotec (2 mentions)

2 protocols using anti ifn γ pe cyanine7 clone xmg1

1

Quantifying T Cell Proliferation and Cytokine Profiles

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To analyze the proliferation of T cells, CFSE (eBioscience Inc., San Diego, CA, USA) was used. Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1, BioLegend), respectively. Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11, BioLegend), respectively, with anti-CD4-FITC (clone GK1.5, BioLegend) after treatment with the Fixation Buffer (#420801, BioLegend) and the Intracellular Staining Perm Wash Buffer (#421002, BioLegend). Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotech) and analyzed with FlowJo (Tomy Digital Biology, Tokyo, Japan).
Kodama N., Okada H., Hachisu M., Ando M., Ito N., Nagata K., Katagiri M., Yasuda Y., Hiroki I., Yashiro T., Ichihara G., Yamamoto M, & Nishiyama C. (2023). A rose flavor compound activating the NRF2 pathway in dendritic cells ameliorates contact hypersensitivity in mice. Frontiers in Nutrition, 10, 1081263.
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2

Multiparametric Flow Cytometry Analysis

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To analyze the proliferation of T cells, CFSE (eBioscience Inc., San Diego, CA, USA) was used. Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1, BioLegend), respectively. Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11, BioLegend), respectively, with anti-CD4-FITC (clone GK1.5, BioLegend) after treatment with the Fixation Buffer (#420801, BioLegend) and the Intracellular Staining Perm Wash Buffer (#421002, BioLegend). Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotech) and analyzed with FlowJo (Tomy Digital Biology, Tokyo, Japan).
Kodama N., Okada H., Hachisu M., Ando M., Ito N., Nagata K., Yasuda Y., Hiroki I., Yashiro T., Ichihara G., Yamamoto M., & Nishiyama C. (2022). A rose flavor compound activating the NRF2 pathway in dendritic cells ameliorates contact hypersensitivity in mice.
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