FOXA1 stable knockdown pGIPZ lentiviral shRNAmir construct (Clone ID#V2LHS_16780) was obtained from Open Biosystems. IL-8 stable knockdown construct was generated by inserting oligos (CGAACTTTAATTTCAGGAA) to the pLKO.1 backbone. FOXA1 overexpression construct was generated by cloning full-length FOXA1 into pQCXIH (Clontech). IL-8 promoter with FOXA1 binding region was amplified by PCR from HEK293T genomic DNA and cloned into pGL4.1[luc2] luciferase reporter vector (Promega).
Stable knockdown pgipz lentiviral shrnamir construct
Manufactured by Thermo Fisher Scientific
The Stable knockdown pGIPZ lentiviral shRNAmir construct is a laboratory tool designed for the knockdown of target genes in mammalian cells. It utilizes a lentiviral vector system to deliver short hairpin RNA (shRNA) molecules that can induce RNA interference (RNAi) and suppress the expression of specific genes.
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Lab products found in correlation
2 protocols using stable knockdown pgipz lentiviral shrnamir construct
1
FOXA1 and IL-8 Regulation in Cancer Cells
2
FOXA1 and IL-8 Regulation in Cancer Cells
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