Male C57BL/6 mice (aged 8 weeks, weighing 22–25 g) were obtained from SLC Japan (Shizuoka, Japan). Mice were housed at 22–24°C with food and water available ad libitum. Animals were divided into three groups as follows; sham, LISW-Vehicle, and LISW-SSRI. Sertraline (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was selected as the SSRI and administered at 15 mg/kg to the SSRI group by oral gavage for 14 days after LISW exposure (23 (link)–26 (link)). The dose of Sertraline was decided following a review of the literature (23 (link), 24 (link)). Mice in the Vehicle and sham groups were administered distilled water instead of Sertraline by oral gavage for 14 days. Sertraline or distilled water was administered to mice 24 h after LISW exposure on day 1. The sham-operated group underwent anesthesia and shaving of the head, but no LISW was applied (Figure 1 ). In this study, the mortality rate of the mice was zero. The study design was approved by the Ethics Committee of National Defense Medical College at the time of study initiation (approval number: 16010).
Sertraline
Manufactured by Fujifilm
Sourced in Japan
Sertraline is a laboratory equipment product designed for conducting chemical analysis and testing. It is a versatile instrument that can be used in various research and development applications.
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2 protocols using sertraline
1
LISW and Sertraline Treatment in Mice
2
Liver Microsome Preparation and Characterization
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Midazolam, rizatriptan, phenylephrine and sertraline were purchased from Wako Pure Chemical Industries (Osaka, Japan). Sumatriptan and S-citalopram were purchased from Tokyo Chemical Industry (Tokyo, Japan). Imipramine was purchased from Nakalai Tesque (Kyoto, Japan). Eletriptan and fatty acid-free human serum albumin (HSA) were obtained from Sigma-Aldrich (St. Louis, MO). All other chemicals and reagents were analytical-grade products from commercial sources. Pooled HLMs (50 donors, #PLo50BA), CD-1 mouse liver microsomes, SD rat liver microsomes and beagle dog liver microsomes were purchased from Thermo Fisher Scientific (Waltham, MA). Pooled HLMt (5 donors, #1110152) was purchased from Sekisui XenoTech.
(Kansas City, KS). An MAO expression system (Supersomes: MAO-A, MAO-B and control) was purchased from Corning (Corning, NY). Individual HLMs and HLMt were prepared according to the previously reported method (13) . Human liver blocks were obtained from Human and Animal Bridging Research Organization (Chiba, Japan). The study protocol was approved by the ethical committees of the School of Medicine in Kanazawa University. Individual HLMs and HLMt were independently prepared to prevent loss derived from sequential purification.
(Kansas City, KS). An MAO expression system (Supersomes: MAO-A, MAO-B and control) was purchased from Corning (Corning, NY). Individual HLMs and HLMt were prepared according to the previously reported method (13) . Human liver blocks were obtained from Human and Animal Bridging Research Organization (Chiba, Japan). The study protocol was approved by the ethical committees of the School of Medicine in Kanazawa University. Individual HLMs and HLMt were independently prepared to prevent loss derived from sequential purification.
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