Gfap rabbit polyclonal antibody

SFN was purchased from Aladdin (China). The CCK-8 assay kit was purchased from Dojindo (Japan). FITC Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (USA). DNase I was purchased from Sigma (USA). The TUNEL apoptosis detection kit and DAPI were purchased from Beyotime (China). DMEM/F12 medium, fetal bovine serum (FBS), 25% (w/v) trypsin-EDTA, Phosphate Buffered Saline (PBS), goat serum, Alexa fluor 594 labeled goat anti-chicken secondary antibodies and Alexa fluor 488 labeled goat anti-rabbit secondary antibodies, RIPA lysis buffer, protein phosphatase inhibitors, BCA protein assay kit, and the ECL reagent kit were purchased from Thermo Scientific (USA). Caspase-3 and cleaved Caspase-3 rabbit polyclonal antibodies, SB203580, p38 MAPK, and phospho-p38 MAPK rabbit mAb were purchased from Cell Signaling Technology (USA). GFAP chicken polyclonal antibody, GFAP rabbit polyclonal antibody, and AQP4 rabbit polyclonal antibody were purchased from Abcam (USA). Beta tubulin rabbit polyclonal antibody and horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were purchased from Proteintech (USA).
SFN was dissolved in dimethyl sulfoxide (DMSO) to a storage concentration of 100 mM. SB203580 was dissolved in dimethyl sulfoxide (DMSO) to a storage concentration of 10 mM. 0.01%(v/v) DMSO was used as vehicle.

Brains were dissected from each mouse and subsequently halved. The right hemisphere was embedded using optimal cutting temperature (OCT) media. After freezing, the block was sectioned using a cryostat at 60 μm and mounted on positively charged slides. Fluorescent staining was conducted using commercially available reagents (Abcam, Waltham, MA, USA). Glial fibrillary acidic protein (GFAP) rabbit polyclonal antibody (Abcam, Cambridge, MA, USA) was diluted to 1:2000 and incubated on each slide for 1–2 h. Sections were counterstained with DAPI (Thermo Scientific, Waltham, MA, USA) in a sealed box protected from light for 8 min. Once dry, ProLong Gold antifade reagent (Invitrogen, Waltham, MA, USA) was applied to the tissue. Once complete, slides were stored at room temperature.