The Nb.BbvCI nicking endonuclease and Klenow fragment (3′–5′ exo-) polymerase (KF polymerase) were acquired from New England Biolabs (Beijing, China). TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 8.0), deoxynucleotides (dNTPs), 6× loading buffer, acryl/bis 30% solution (29 : 1), ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sangon Biotechnology Co. Ltd (Shanghai, China). Thioflavin T (ThT) was acquired from Sigma-Aldrich (St. Louis, USA). GoldView I was purchased from SBS Genetech (Beijing, China). The 20-bp DNA Ladder (Dye Plus) was purchased from TaKaRa Biotech (Dalian, China). Human serum was from the First Affiliated Hospital of Chongqing Medical University. All oligonucleotides used in this study were synthesized by Sangon Biotech Inc. (Shanghai, China), and the detailed oligonucleotide sequences are listed in Table S1.† During the design of P-HP, the website https://sg.idtdna.com/calc/analyzer was employed to predict the secondary structure. Ultrapure water obtained from a Millipore water purification system (18.2 MΩ, Milli-Q, Millipore) was used to prepare all the solutions in this experiment.
Klenow fragment 3 5 exo polymerase kf polymerase
Manufactured by New England Biolabs
Sourced in China
Klenow fragment (3'–5' exo-) polymerase (KF polymerase) is a DNA polymerase enzyme derived from the Escherichia coli DNA polymerase I. It catalyzes the 5' to 3' synthesis of DNA from a primed template.
Automatically generated - may contain errors
Lab products found in correlation
20 bp dna ladder dye plus, by Takara Bio (2 mentions)
Te buffer, by Sangon (2 mentions)
Milli q, by Merck Group (1 mentions)
Thioflavin t tht, by Merck Group (1 mentions)
Nb bbvci nicking endonuclease, by New England Biolabs (1 mentions)
N n n n tetramethylethylenediamine temed, by Sangon (1 mentions)
Ammonium persulfate, by Sangon (1 mentions)
Water purification system, by Merck Group (1 mentions)
Ammonium persulfate aps, by Sangon (1 mentions)
Dna loading buffer, by Sangon (1 mentions)
Hplc purified oligonucleotides, by Sangon (1 mentions)
Goldview, by Solarbio (1 mentions)
2 protocols using klenow fragment 3 5 exo polymerase kf polymerase
1
Optimized Nicking Endonuclease-Mediated DNA Amplification
2
Purification and Characterization of Oligonucleotides
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HPLC-purified oligonucleotides used in this research (as listed in Table S1) were supplied by Sangon Biotech Inc. (Shanghai, China) and fully dissolved in an ice box with 1 × TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) at a storage concentration of 10 μM.
The 1 × TE buffer, 10 mM deoxynucleotide triphosphates (dNTPs), 6 × DNA loading buffer, 30% acrylamide/bis solution, and ammonium persulfate (APS) were obtained from Sangon Biotechnology Co. Ltd (Shanghai, China).
N,N,N′,N′-Tetramethylethylenediamine (TEMED) and 20 bp DNA Ladder (Dye Plus) used for polyacrylamide gel electrophoresis was acquired from TaKaRa Biotech (Dalian, China). GoldView was acquired from Solarbio LIFE SCIENCES (Beijing, China). ThT was purchased from BBI LIFE SCIENCES CORPORATION (Shanghai, China) and dissolved in ultrapure water to 100 μM for fluorescent measurements. Nb.BbvCI endonuclease and Klenow fragment (3′-5′ exo-) polymerase (KF polymerase) used in this study were provided by New England Biolabs (Beijing, China). Ultrapure water used for solution preparation was obtained from a Millipore water purification system with a resistivity of 18.2 MΩ cm. Human serum samples for the recovery experiment were obtained from the First Affiliated Hospital of Chongqing Medical University.
The 1 × TE buffer, 10 mM deoxynucleotide triphosphates (dNTPs), 6 × DNA loading buffer, 30% acrylamide/bis solution, and ammonium persulfate (APS) were obtained from Sangon Biotechnology Co. Ltd (Shanghai, China).
N,N,N′,N′-Tetramethylethylenediamine (TEMED) and 20 bp DNA Ladder (Dye Plus) used for polyacrylamide gel electrophoresis was acquired from TaKaRa Biotech (Dalian, China). GoldView was acquired from Solarbio LIFE SCIENCES (Beijing, China). ThT was purchased from BBI LIFE SCIENCES CORPORATION (Shanghai, China) and dissolved in ultrapure water to 100 μM for fluorescent measurements. Nb.BbvCI endonuclease and Klenow fragment (3′-5′ exo-) polymerase (KF polymerase) used in this study were provided by New England Biolabs (Beijing, China). Ultrapure water used for solution preparation was obtained from a Millipore water purification system with a resistivity of 18.2 MΩ cm. Human serum samples for the recovery experiment were obtained from the First Affiliated Hospital of Chongqing Medical University.
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