Collagenases 4

Manufactured by Worthington

Collagenases IV is a laboratory reagent used to enzymatically digest collagen, a structural protein found in various tissues. It is commonly utilized in cell culture applications to facilitate the isolation and extraction of cells from their extracellular matrix.

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Close spelling variants of this product

Collagenase Collagenase IV Collagenase I and IV Collagenase IV (LS004188) Collagenase IV (CLS-4, Collagenase I-IV Collagenase type I and IV Ultrapure collagenase IV Collagenase type II and IV Collagenase type IV solution

2 protocols using collagenases 4

Dissociation of Fetal Liver and Yolk Sac

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Dissected fetal liver (FL) and YS, at indicated embryonic stages, were finely chopped and the obtained fragments were digested in a mix of Collagenases IV (2 mgml⁻¹, Worthington) and DNase I (200 Uml⁻¹, Calbiochem) at 37 °C with gentle agitation for 15 min. The dissociated cells were centrifuged at 300 g for 6 min and resuspended in PBS supplemented with 10% FBS.

Neo W.H., Meng Y., Rodriguez-Meira A., Fadlullah M.Z., Booth C.A., Azzoni E., Thongjuea S., de Bruijn M.F., Jacobsen S.E., Mead A.J, & Lacaud G. (2021). Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors. Nature Communications, 12, 7019.

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Fetal Lung Cell Isolation Protocol

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Dissected FL at indicated fetal stage were digested in a mix of Collagenases IV (2 mgml^-1, Worthington) and DNase I (200 Uml^-1, Calbiochem) at 37 ºC for 15 min. The dissociated cells were centrifuged at 300 g for 6 min and resuspended in 10% FBS/PBS.

Neo W.H., Booth C.A., Azzoni E., Chi L., Delgado-Olguín P., de Bruijn M.F., Jacobsen S.E, & Mead A.J. (2018). Cell-extrinsic hematopoietic impact of Ezh2 inactivation in fetal liver endothelial cells. Blood, 131(20), 2223-2234.

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