Fresh or thawed (from frozen stock) human brain blocks were embedded in Tissue-Tek® O.C.T. Compound and frozen at −80oC overnight. Before sectioning at 20–30 μm thickness using a cryostat (Leica), frozen blocks were changed to −20oC for 2 h to soften tissue for sectioning. Cryo-sections were then directly mounted on MAS-GP™ Adhesion Microscope Slides (Matsunami) and stored at 4oC before staining. For 3,30-diaminobenzidine (DAB) staining, cryo-sections were equilibrated in PBS containing 0.3% Triton-X100 (PBST) for 30 min followed by blocking endogenous peroxidase activity and antibody non-specific binding, ffor 1 h respectively. Primary antibodies were diluted in PBST and incubated with sections at 4°C overnight. After 3 washes with PBST, sections were then incubated with biotinylated secondary antibodies for 1 h. The immunoreactive products were visualized by incubating with 1) DAB containing nickel ammonium sulfate as an enhancing reagent or 2) Vina Green chrome (Biocare Medical). Stained sections were observed using an Axioskop 2 (Zeiss).
Mas gp adhesion microscope slides
Manufactured by Matsunami
The MAS-GP™ Adhesion Microscope Slides are a product designed for microscopy applications. They provide a specialized surface for the adhesion and observation of samples under a microscope. The slides are made with a proprietary coating that enhances the adherence of materials to the slide's surface, facilitating effective sample preparation and analysis.
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Lab products found in correlation
2 protocols using mas gp adhesion microscope slides
1
Cryopreserved Human Brain Tissue Analysis
2
Immunohistochemical Analysis of Alzheimer's Disease
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Human Alzheimer’s disease cortex was embedded into Tissue-Tek OCT Compound and frozen at −80°C overnight. Before sectioning at 20–30-µm thicknesses using a cryostat (Leica), deep-frozen blocks were changed to −20°C for 2 h to soften tissue for sectioning. Cryo-sections were then mounted on MAS-GP Adhesion Microscope Slides (Matsunami) and stored at 4°C before staining. Cryo-sections were equilibrated in PBS containing 0.03% Triton X-100 (PBS-TX) for 30 min followed by blocking with 5% milk in PBS-TX for 1 h. Primary antibodies were diluted in PBS-TX and added at 4°C overnight. After a wash with PBS-TX, sections were incubated with Alexa-488 or Alexa-647 conjugated secondary antibodies for 1 h followed by PBS-TX wash. Micrographs were taken using a DMi8 Widefield microscope (Leica).
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