The para n-embedded tissue samples were depara nized, rehydrated, and heated in antigen retrieval Citra solution (pH 6; BioGenex Laboratories, San Ramon, CA, USA). After blocked with 2% bovine serum album in PBS buffer for 1 hour, the slides were stained with the CD8 (Biorbyt, San Francisco, CA), PD-1 (Thermo Fisher, Waltham, MA, USA), TIM-3 (Bio-Rad, Hercules, California, USA) primary antibody at 4℃overnight. The next day, incubated the slides with uorochrome-conjugated secondary antibodies (Alexa 594 anti-rabbit, Alexa 488 anti-mouse, Alexa 647 anti-Rat, Invitrogen, Carlsbad, CA, USA). After rinsing with PBS, slides were mounted with Vectashield antifade mounting medium with 4', 6-diamidino-2-phenylindole (DAPI).
Tim 3
Manufactured by Bio-Rad
Sourced in United States
The TIM-3 is a laboratory instrument manufactured by Bio-Rad. It is designed to measure and analyze the expression of the TIM-3 protein, which plays a role in the regulation of the immune system. The TIM-3 instrument provides researchers with the necessary tools to study the function and expression of this protein in various biological samples.
Automatically generated - may contain errors
2 protocols using tim 3
1
Multiplex Immunohistochemistry for Immune Markers
2
Multiplex Immunohistochemistry for Immune Markers
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The para n-embedded tissue samples were depara nized, rehydrated, and heated in antigen retrieval Citra solution (pH 6; BioGenex Laboratories, San Ramon, CA, USA). After blocked with 2% bovine serum album in PBS buffer for 1 hour, the slides were stained with the CD8 (Biorbyt, San Francisco, CA), PD-1 (Thermo Fisher, Waltham, MA, USA), TIM-3 (Bio-Rad, Hercules, California, USA) primary antibody at 4℃overnight. The next day, incubated the slides with uorochrome-conjugated secondary antibodies (Alexa 594 anti-rabbit, Alexa 488 anti-mouse, Alexa 647 anti-Rat, Invitrogen, Carlsbad, CA, USA). After rinsing with PBS, slides were mounted with Vectashield antifade mounting medium with 4', 6-diamidino-2-phenylindole (DAPI).
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