The largest database of trusted experimental protocols

2 protocols using ps100

1

Central Pathology Review of Soft Tissue Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
The central pathology review was performed conjointly by 3 neuropathologists (ATE, PV, EL), a pathologist expert in soft tissue tumors (FL), and a pathologist expert in vascular lesions (MW). For the CNS cases, additional immunohistochemical stainings were performed on paraffin-embedded sections including: CD34 (1:40, clone QBEnd10, Dako, Glostrup, Denmark), smooth muscle actin (SMA) (1:1500, clone 1A4, Dako, Glostrup, Denmark), Desmin (1:400, clone D33, Dako, Glostrup, Denmark), h-caldesmon (1:100, clone h-CALD, Santa Cruz Biotechnology, Dallas, USA), PS100 (1:6000, polyclonal, Dako, Glostrup, Denmark), GFAP (1:200, clone 6F2, Dako, Glostrup, Denmark), neurofilament (1:25, clone 2F11, Dako, Glostrup, Denmark), STAT6 (1:200, clone YE361, Abcam, Cambridge, UK), SSTR2a (1:200, clone UMB1, Abcam, Cambridge, UK) and EMA (1:200, clone GM008, Dako, Glostrup, Denmark). All immunohistochemical stainings were performed in an automatic closed immunostainer (Omnis automate). Orcein staining was also performed.
+ Open protocol
+ Expand
2

Immunocytochemical Profiling of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SRH imaging, the sample were recovered from between the coverslips and fixed in 4% formalin for 24 hours. The specimens were then included in a conventional pathology workflow comprising paraffin embedding, sectioning and HE staining. Immunochemistry was performed on the Benchmark Ventana autostainer (Ventana Medical Systems SA, Illkirch, France) with routinely used antibodies targeting AE1-AE3 (Polyclonal, Roche), Bcl6 (PG-B6p, Dako), CD10 (56C6, Dako), CD20 (L26, Cliniscience), CD3 (Polyclonal, Dako), CD56/NCAM (123C3, Roche), CDX2 (DAK-CDX2, Dako), Chromogranin (DAK-A3, Dako), CK20 (Ks20.8, Dako), CK5/6 (DS/16B4, Dako), CK7 (OV.TL12/30, Menarini), EGFR (3C6, Roche), EMA (E29, Dako), Filamin (PM6/317, Interchim), FSH (Polyclonal, Roche), GATA3 (L50-823, Roche), GFAP (6F2, Dako), IDH1 R132H (H09, Clinisciences), Ki67 (Mib-1, Dako), MUM1 (MUM1P, Dako), NeuN (A60, Millipore), Neurofilament (2F11, Sigma-Aldrich), OLIG2 (EP112, Diagomix), P63 (4A4, Roche), PS100 (Polyclonal, Dako), RP (1/E2, Roche), SSTR2 (UMB1, Abcam), Synaptophysin (DAK-SYNAP, Dako), TTF1 (8G7G3/1, Dako). The slides were scanned using the Hamamatsu Nanozoomer system 2.0-HT and the presented images were obtained using the NDP.view2 viewing software (ver 2.6.17).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!