Sections from tumors were cut into 4 μm in thickness and deparaffinized in xylene for 10 min. The slides were immersed in 3% H2O2 for 20 min to block the endogenous peroxidase and were blocked in goat serum blocking solution for 30 min. After being incubated at 4 °C overnight with primary antibodies, the slides were incubated with secondary HRP-conjugated antibodies (Thermo Fisher Scientific) for 30 min at RT. The primary antibodies were as follows: F4/80 (Cell Signaling Technology, 70076S, 1:200), iNOS (Abcam, ab3523, 1:50), CD206 (proteintech, 18,704–1-AP, 1:200), CD4 (Cell Signaling Technology, 25229S, 1:200), CD8 (Cell Signaling Technology, 98941S, 1:200), Granzyme B (NOVUS, NB100–684, 1:200), and PD-1 (Abcam, ab214421, 1:200). IHC stainings were examined with microscopy.
Granzyme b
Manufactured by Novus Biologicals
Granzyme B is a serine protease enzyme that is involved in the process of apoptosis, or programmed cell death. It is expressed in the cytotoxic granules of natural killer cells and cytotoxic T cells, and plays a role in the immune system's ability to eliminate infected or cancerous cells.
Automatically generated - may contain errors
2 protocols using granzyme b
1
Immunophenotyping of Tumor-Infiltrating Immune Cells
2
Immunohistochemical Analysis of Tumor Samples
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Sections from tumors were cut into 4 μm in thickness and depara nized in xylene for 10 min. The slides were immersed in 3% H 2 O 2 for 20 min to block the endogenous peroxidase and were blocked in goat serum blocking solution for 30 min. After being incubated at 4 °C overnight with primary antibodies, the slides were incubated with secondary HRP-conjugated antibodies (Thermo Fisher Scienti c) for 30 min at RT. The primary antibodies were as follows: F4/80 (Cell Signaling Technology), iNOS (Abcam), CD206 (proteintech), CD4 (Cell Signaling Technology), CD8 (Cell Signaling Technology), Granzyme B (NOVUS), and PD-1 (Abcam). IHC stainings were examined with microscopy.
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