Rifampin (rif, Fisher Scientific, BP26795) 0.4 grams was dissolved in 40 mL methanol (HPLC grade, Fisher Scientific), resulting in 10,000 ppm rif stock solution, filter-sterilized (0.2 micron nylon filter, Fisher Scientific), and stored refrigerated (4°C) in the darkness for no longer than one month. Rifampin was added to the cooled autoclaved Difco TM tryptic soy agar (TSA, Becton, Dickinson, and Co) or Bacto TM tryptic soy broth (TSB, Becton, Dickinson, and Co.) in order to yield 100 ppm final rifampin concentration, such as 0.1 mL rif stock to 10 mL TSB tube, or 10 mL rif stock to 1,000 mL TSA medium.
Bp26795
Manufactured by Thermo Fisher Scientific
The BP26795 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to provide a stable and reliable platform for various laboratory applications. Detailed specifications and intended use are not available for this particular product.
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2 protocols using bp26795
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Preparation of Rifampin Stock Solution
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Preparation of Rifampin Stock Solution
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0.4 grams of Rifampin (Fisher Scientific, BP26795) was dissolved in 40 mL methanol (HPLC grade, Fisher Scientific), filter-sterilized (0.2 micron nylon filter, Fisher Scientific) to prepare sterile 10,000 ppm stock solution, and stored refrigerated (4 °C) in the darkness for no longer than a month. Rifampin stock solution was added to the cooled autoclaved Difco TM tryptic soy agar (TSA, Becton, Dickinson, and Co) or Bacto TM tryptic soy broth (TSB, Becton, Dickinson, and Co.) to yield 100 ppm final Rifampin concentration, such as 0.1 mL rif stock to 10 mL TSB tube, or 10 mL rif stock to 1,000 mL TSA medium.
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