The digestion medium for tissue disaggregation and cell detachment contained a Hank's Balanced Salt Solution (HBSS) with calcium and magnesium (HBL03-Caisson, Smith eld, USA), supplemented with 200 U/mL collagenase type I (C0130-Sigma-Aldrich, St. Louis, USA), 1.2 U/mL dispase II (D4693-Sigma-Aldrich, St. Louis, USA), 0.01 mg/mL soybean trypsin inhibitor (STI) (29129-Chem Cruz, Dallas, USA), 1.25 mg/mL bovine serum albumin (BSA) (B005-Caisson, Smith eld, USA) and 0.1 mM Dithiothreitol (DTT) (A2948-PanReac, Barcelona, Spain). The solution was freshly prepared, ltered with 0.2 μM nylon membranes, stored at 4°C and used within the next 24 hours.
A2948
Manufactured by ITW Reagents
Sourced in Spain
The A2948 is a laboratory equipment product manufactured by ITW Reagents. It is a device designed for specific laboratory functions. No further details can be provided in an unbiased and factual manner without the risk of extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a2948
1
Tissue Disaggregation and Cell Detachment
2
Tissue Disaggregation and Cell Detachment
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The digestion medium for tissue disaggregation and cell detachment contained a Hank's Balanced Salt Solution (HBSS) with calcium and magnesium (HBL03-Caisson, Smith eld, USA), supplemented with 200 U/mL collagenase type I (C0130-Sigma-Aldrich, St. Louis, USA), 1.2 U/mL dispase II (D4693-Sigma-Aldrich, St. Louis, USA), 0.01 mg/mL soybean trypsin inhibitor (STI) (29129-Chem Cruz, Dallas, USA), 1.25 mg/mL bovine serum albumin (BSA) (B005-Caisson, Smith eld, USA) and 0.1 mM Dithiothreitol (DTT) (A2948-PanReac, Barcelona, Spain). The solution was freshly prepared, ltered with 0.2 μM nylon membranes, stored at 4°C and used within the next 24 hours.
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