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Total gangliosides ga

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Total gangliosides (GA) is a laboratory product that represents the total content of gangliosides, which are a class of glycosphingolipids found in cell membranes. This product can be used for analytical and research purposes to measure the overall ganglioside composition in various biological samples.

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Lab products found in correlation

Mini extruder set, by Avanti Polar Lipids (2 mentions) Pc membrane, by Cytiva (2 mentions) Whatman, by Cytiva (2 mentions)

Close spelling variants of this product

Ganglioside (2 citations)

2 protocols using total gangliosides ga

1

Endosomal Membrane Lipid Liposomes

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Phospholipids were selected based on phospholipids typically found in endosomal membrane except for charged phospholipids22 (link). POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), POPE (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine), cholesterol, and total gangliosides (GA) (Avanti Polar Lipids) were dissolved in chloroform-methanol (2:1) at a final concentration of 10 mM. Liposomes with 3 different lipid compositions were prepared: A) 95 mol % POPC and 5 mol % GA; B) 66 mol % POPC, 13 mol % POPE, 16 mol % cholesterol and 5 mol % GA; C) 42 mol % POPC, 13 mol % POPE, 40 mol % cholesterol and 5 mol % GA. The solvent was removed by evaporation; first using a stream of argon gas and second, high vacuum for 1 hour. The lipid film was re-suspended in KHE buffer, processed using 21 freeze-thaw cycles, and extruded at room temperature through 100 nm PC membrane (Whatman) using a mini-extruder set (Avanti Polar Lipids). Liposomes were stored at 4°C and used within 1 to 5 days.
Chlanda P., Mekhedov E., Waters H., Schwartz C.L., Fischer E.R., Ryham R.J., Cohen F.S., Blank P.S, & Zimmerberg J. (2016). The hemifusion structure induced by Influenza virus haemagglutinin is determined by physical properties of the target membranes. Nature microbiology, 1(6), 16050.
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2

Endosomal Membrane Lipid Liposomes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospholipids were selected based on phospholipids typically found in endosomal membrane except for charged phospholipids22 (link). POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), POPE (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine), cholesterol, and total gangliosides (GA) (Avanti Polar Lipids) were dissolved in chloroform-methanol (2:1) at a final concentration of 10 mM. Liposomes with 3 different lipid compositions were prepared: A) 95 mol % POPC and 5 mol % GA; B) 66 mol % POPC, 13 mol % POPE, 16 mol % cholesterol and 5 mol % GA; C) 42 mol % POPC, 13 mol % POPE, 40 mol % cholesterol and 5 mol % GA. The solvent was removed by evaporation; first using a stream of argon gas and second, high vacuum for 1 hour. The lipid film was re-suspended in KHE buffer, processed using 21 freeze-thaw cycles, and extruded at room temperature through 100 nm PC membrane (Whatman) using a mini-extruder set (Avanti Polar Lipids). Liposomes were stored at 4°C and used within 1 to 5 days.
Chlanda P., Mekhedov E., Waters H., Schwartz C.L., Fischer E.R., Ryham R.J., Cohen F.S., Blank P.S, & Zimmerberg J. (2016). The hemifusion structure induced by Influenza virus haemagglutinin is determined by physical properties of the target membranes. Nature microbiology, 1(6), 16050.
+ Open protocol
+ Expand

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