SW480 (Male) were cultured in Leibovitz’s L-15 medium (Gibco) supplemented with 10% FBS (Thermo Fisher) at 37°C 5% CO2. Original cultures were seeded at 150k cells per well in TC-treated 6-well plates (CellTreat Scientific Products) and passaged every 5 days at a ratio of 1 to 4.
Tc treated 6 well plates
Manufactured by Celltreat
The TC-treated 6-well plates are a type of cell culture vessel designed for in vitro studies. Each plate has six individual wells that have been treated to promote cell attachment and growth. The plates are manufactured to provide a consistent and standardized surface for cell culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (2 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Opti mem medium, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using tc treated 6 well plates
1
Propagating SW480 Colorectal Cancer Cells
2
Culturing HCT116 Cells in McCoy's 5A
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HCT116 (Male) were cultured in McCoy’s 5A medium (Gibco) supplemented with 10% FBS (Thermo Fisher) at 37°C 5% CO2. Original cultures were seeded at 150k cells per well in TC-treated 6-well plates (CellTreat Scientific Products) and passaged every 5 days at a ratio of 1 to 10.
3
Culturing HT29 Cells in McCoy's 5A
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HT29 (Female) were cultured in McCoy’s 5A medium (Gibco) supplemented with 10% FBS (Thermo Fisher) at 37°C 5% CO2. Original cultures were seeded at 150k cells per well in TC-treated 6-well plates (CellTreat Scientific Products) and passaged every 5 days at a ratio of 1 to 5.
4
HIEC Cell Culture Conditions
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HIEC cells were cultured in OptiMEM medium (Gibco) supplemented with 4% Premium quality FBS (Wisent), 20mM HEPES, 10mM GlutaMAX (Gibco), and 10ng/ml EGF at 37°C 5% CO2. Original cultures were seeded at 300k cells per well in TC-treated 6-well plates (CellTreat Scientific Products) and passaged every 5 days at a ratio of 1 to 3.
5
Cultivation of MC38 Colorectal Adenocarcinoma
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Mouse colorectal adenocarcinoma MC38 (Male) cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS (Thermo Fisher), 2mM glutamine, 0.1mM nonessential amino acids, 1mM sodium pyruvate, and 10mM HEPES at 37°C 5% CO2. Original cultures were seeded at 150k cells per well in TC-treated 6-well plates (CellTreat Scientific Products) and passaged every 3 days at a ratio of 1 to 5.
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