Sune1

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

SUNE1 is a cell culture incubator designed for maintaining optimal environmental conditions for cell growth and proliferation. It features precise temperature, humidity, and CO2 control to support a wide range of cell types. The incubator provides a stable and protected environment for cell cultures during long-term experiments or culturing.

Automatically generated - may contain errors

Lab products found in correlation

C666 1, by National Collection of Authenticated Cell Cultures (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Cne 1, by National Collection of Authenticated Cell Cultures (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Mir 762 mimic, by RiboBio (1 mentions) Mir 762 inhibitor, by RiboBio (1 mentions) Cne 2, by National Collection of Authenticated Cell Cultures (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) P msk1, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Anti traf2, by Cell Signaling Technology (1 mentions) Cyclin b, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) P creb, by Cell Signaling Technology (1 mentions) Cyclin a, by Cell Signaling Technology (1 mentions)

8 protocols using sune1

Transfection of Nasopharyngeal Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

NPC cell lines (C666-1 [contains completed EBV genome], SUNE1, 5-8F, and 6-10B) and nasopharyngeal epithelial cell NP69 were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS; Invitrogen Corp, Grand Island, NY, USA) in a humidified incubator at 37°C with 5% CO 2 .
NPC cells were transfected with miR-762 mimic or miR-762 inhibitor (Ribobio, Guangdong, China) to regulate the expression level of miR-762. Corresponding negative control (mimic NC and inhibitor NC) was also transfected and untreated cells were set as a control group. Cell transfection was conducted with the help of the Lipofectamine 3000 (Invitrogen, Shanghai, China).

Bao L.H., Ji K., Li D., Liu S.S., Song Z.Y, & Xia G.H. (2021). The biological function and diagnostic value of miR-762 in nasopharyngeal carcinoma. Journal of the Chinese Medical Association : JCMA, 84(5).

+ Open protocol

+ Expand

Cell culture protocol for NPC and NP69

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five NPC cell lines (C666-1, SUNE-1, CNE1, CNE2 and HNE-1) and the normal nasopharyngeal cell line NP69 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cells were maintained in DMEM, and other cells were cultured in RPMI1640 (GIBCO, Grand Island, NY, USA) supplemented with 10% FBS. Cells were cultured in a humidified incubator in a 5% CO₂ atmosphere at 37 °C.

Wu J.H., Tang J.M., Li J, & Li X.W. (2018). Upregulation of SOX2-activated lncRNA ANRIL promotes nasopharyngeal carcinoma cell growth. Scientific Reports, 8, 3333.

+ Open protocol

+ Expand

Culturing Nasopharyngeal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four different NPC cell lines (5-8F, 6-10B, SUNE1 and C666-1) and normal nasopharyngeal-derived epithelial cells (NP69) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). NPC cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco Laboratories, NY, U.S.A.) medium containing 10% fetal bovine serum (FBS, Gibco Laboratories), while NP69 cells were maintained in Keratinocyte-SFM medium (K-SFM, Gibco Laboratories). All cells were maintained in 5% CO₂ at 37°C in a humid atmosphere.

Liu H., Cheng Y., Xu Y., Xu H., Lin Z., Fan J, & Lang J. (2019). The inhibition of tumor protein p53 by microRNA-151a-3p induced cell proliferation, migration and invasion in nasopharyngeal carcinoma. Bioscience Reports, 39(10), BSR20191357.

+ Open protocol

+ Expand

Nasopharyngeal Cell Lines Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human immortalized nasopharyngeal epithelial cell NP460 and nasopharyngeal carcinoma cell lines, including CNE1, CNE2, C666-1, HNE1, HNE3, HK1, HNE2, HONE1, and SUNE1, were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai). The 293T cells for virus infection was purchased from American Type Culture Collection (ATCC, Manassas, VA). All cells were cultured by following the standard protocols. The primary antibodies including anti-TRAF2, p-MSK1, p-CREB, cyclin D1, CDK4, cyclin A, CDK2, cyclin B, CDK1, p27, p21, β-actin, Ki67, cleaved PARP, cleaved caspase-3, and secondary HRP-conjugated goat anti-mouse/rabbit IgG antibodies were products of Cell Signaling Technology (Danvers, MA). The SuperSignal™ West Dura Extended Duration Substrate was a product of Thermo Fisher Scientific (Waltham, MA).

Zhu H., Ding W., Wu J., Ma R., Pan Z, & Mao X. (2020). TRAF2 Knockdown in Nasopharyngeal Carcinoma Induced Cell Cycle Arrest and Enhanced the Sensitivity to Radiotherapy. BioMed Research International, 2020, 1641340.

+ Open protocol

+ Expand

Cultivation of Nasopharyngeal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal nasopharyngeal epithelial cells NP69 and NPC cell lines (HK-1, C666-1, 5-8F, and SUNE-1) were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai). All the cells were cultured in the 1640 medium (HyClone, Logan, UT, USA) containing 10% fetal bovine serum. The cell culture was placed in an incubator at 37°C with 5% carbon dioxide. Cells in logarithmic growth phase were used in the experiments.

Shuai M, & Huang L. (2020). High Expression of hsa_circRNA_001387 in Nasopharyngeal Carcinoma and the Effect on Efficacy of Radiotherapy. OncoTargets and therapy, 13, 3965-3973.

+ Open protocol

+ Expand

Cultivation of Nasopharyngeal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human immortalized nasopharyngeal epithelial cell line NP69 and NPC cell lines (CNE-2Z, HNE-2, C666-1, 5–8F and SUNE-1) were obtained from China Center for Type Culture Collection (Wuhan, China). These six cells were kept in RPMI-1640 or DMEM (High Glucose) medium (Thermo Fisher, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, CA, USA), penicillin (100 U/mL), and streptomycin (100 mg/mL), and were grown in a 5% CO₂ incubator at 37°C. Cisplatin (DDP)-resistant (CNE-2Z-DDP) and radiotherapy-resistant (CNE-2Z-R) cell lines established as previously described.17 (link),18 (link)

Luo X., He X., Liu X., Zhong L, & Hu W. (2020). miR-96-5p Suppresses the Progression of Nasopharyngeal Carcinoma by Targeting CDK1. OncoTargets and therapy, 13, 7467-7477.

+ Open protocol

+ Expand

Culturing Nasopharyngeal Carcinoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

CNE-1 and SUNE-1 cells were purchased from China Center for Type Culture Collection (Wuhan, China). CNE-2 and NP69 were obtained from the Chinese Academy of Science Cell Bank (Shanghai, China). 6-10B was a kindly gift of Prof. Yiwen You from Affiliated Hospital of Nantong University. Four NPC cell lines (CNE1, CNE2, SUNE-1 and 6-10B) were cultured in RPMI medium 1640 (NCS, Hyclone, Invitrogen) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA). The immortalized normal nasopharyngeal epithelial cell line NP69 was cultured in Keratinocyte-SFM medium supplemented with epidermal growth factor. Cultures were maintained at 37 °C under 5% CO2.

Lin C., Li M., Lin N., Zong J., Pan J, & Ye Y. (2022). RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma. BMC Cancer, 22, 549.

+ Open protocol

+ Expand

Cell Culture Protocols for NPC and HEK293T

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two EBV-negative NPC cell lines CNE-1 and SUNE-1 and human embryonic kidney (HEK) 293 T cells were purchased from China Center for Type Culture Collection (Wuhan, China), and one EBV-positive C666–1cell line was kindly presented by Professor Hong lin Chen from the University of Hong Kong. All NPC cells were cultured in RPMI-1640 medium (HyClone; Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) at 37 °C containing 5% CO₂.HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% FBS at 37 °C containing 5% CO₂.

Lin C., Zong J., Lin W., Wang M., Xu Y., Zhou R., Lin S., Guo Q., Chen H., Ye Y., Zhang B, & Pan J. (2018). EBV-miR-BART8-3p induces epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma cells through activating NF-κB and Erk1/2 pathways. Journal of Experimental & Clinical Cancer Research : CR, 37, 283.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!