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Eol 1

Manufactured by American Type Culture Collection
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The EOL-1 is a cell culture incubator designed for the maintenance and growth of cell lines. It provides a controlled environment with precise temperature, humidity, and carbon dioxide (CO2) regulation to support optimal cell culture conditions.

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Lab products found in correlation

Molm 13, by American Type Culture Collection (4 mentions) Thp 1, by American Type Culture Collection (2 mentions) Hl 60, by American Type Culture Collection (2 mentions) Mv4 11, by American Type Culture Collection (2 mentions) Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions) Molm 14, by American Type Culture Collection (1 mentions) Skm 1, by American Type Culture Collection (1 mentions) Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions) Nomo 1, by American Type Culture Collection (1 mentions) Ku812, by American Type Culture Collection (1 mentions) Mono mac 6, by American Type Culture Collection (1 mentions) Molt 4, by American Type Culture Collection (1 mentions) Meg 01, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Eol 1, by Merck Group (1 mentions) Mv4 11, by Merck Group (1 mentions) Molm 13, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

5 protocols using eol 1

1

Antiproliferative Assays of AML Cell Lines

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The human AML cell lines were obtained from ATCC, except that EOL-1, MOLM-13, MOLM-14, MUTZ-8, NOMO-1 and SKM-1 were obtained from DSMZ (RRID:CVCL_0098). The mouse Ba/F3 cell lines expressing FLT3 mutants ITD, D835Y, ITD plus D835Y, ITD plus F691L were a generous gift from Dr. Michael Andreeff at the MD Anderson Cancer Center, Houston, TX. All lines were STR authenticated at ATCC or DSMZ. Mycoplasma testing was conducted every 2 months using the Universal Mycoplasma Detection Kit from ATCC (catalog# 30–1012K). The last mycoplasma test was within 2 months of the last experiment with any given cell line. The cells were cultured in the medium recommended by the source for each cell line. Antiproliferative assays were performed using Cell Titer 96 Aqueous One Solution Cell Proliferation assay (Promega, catalogue number G3581). Cells were exposed in four replicates to increasing concentrations of drugs for 72 h. Studies using Ba/F3 cells expressing wild type and mutant forms of BTK were performed by Advanced Cellular Dynamics, Inc, Seattle, WA.
Rice W.G., Howell S.B., Zhang H., Rastgoo N., Local A., Kurtz S.E., Lo P., Bottomly D., Wilmot B., McWeeney S.K., Druker B.J, & Tyner J.W. (2022). Luxeptinib (CG-806) Targets FLT3 and Clusters of Kinases Operative in Acute Myeloid Leukemia. Molecular Cancer Therapeutics, 21(7), 1125-1135.
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2

Collection of Human Leukemia Cell Lines

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The human AML tumor cell lines Molm-13 (ACC-554), HL-60 (CCL-240), EOL-1 (ACC-386), HEL (ACC-11), Mono-Mac-6 (ACC-124), THP-1 (TIB-202), and Ma9RAS (MLL/AF9), the CML cell lines KU812 (CRL-2099) and K562 (CCL-243), and the human embryonic kidney epithelial cell line 239T (CRL-3216) were obtained from ATCC and stored in a master cell bank. Cells derived from working cell banks were used for the present study. PBMCs were isolated by Ficoll gradient density centrifugation using Ficoll-Paque (Velizy-Villacoublay, France) from anonymous blood samples collected from healthy donors at a French blood center (Besançon, France). Cord Blood CD34+ cells were purchased from Lymphobank Besançon, France. AML primary cell collection from patients with AML was performed at the time of diagnosis.
Trad R., Warda W., Alcazer V., Neto da Rocha M., Berceanu A., Nicod C., Haderbache R., Roussel X., Desbrosses Y., Daguindau E., Renosi F., Roumier C., Bouquet L., Biichle S., Guiot M., Seffar E., Caillot D., Depil S., Robinet E., Salma Y., Deconinck E., Deschamps M, & Ferrand C. (2022). Chimeric antigen receptor T-cells targeting IL-1RAP: a promising new cellular immunotherapy to treat acute myeloid leukemia. Journal for Immunotherapy of Cancer, 10(7), e004222.
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3

Leukemia Cell Line Cultivation

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The AML cell lines MV4-11, U937, KG-1, THP-1, and other leukemia cell lines HL60, NB4, MOLT4, MEG01 and EOL-1, were purchased from the American type culture collection (ATCC) or Shanghai Cell Bank (Type Culture Collection, Chinese Academy of Sciences). The base medium for MV4-11 and KG-1 cell line is gibco-formulated Iscove's Modified Dulbecco's Medium. All other cell lines were maintained in RPMI-1640 supplemented with 10% FBS, 100 U/mL penicillin, 50 mg/mL streptomycin, and 2 mmol/L glutamine in a humidified CO2 incubator at 37 °C. All cells were passaged for less than 3 months before renewal from frozen, early-passage stocks obtained from the indicated sources.
Wang Y., Zhang L., Tang X., Luo J., Tu Z., Jiang K., Ren X., Xu F., Chan S., Li Y., Zhang Z, & Ding K. (2020). GZD824 as a FLT3, FGFR1 and PDGFRα Inhibitor Against Leukemia In Vitro and In Vivo. Translational Oncology, 13(4), 100766.
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4

Cell Line Cultivation Protocol

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Molm-13, EOL-1 and MV4-11 cell lines were purchased from the American Type Culture Collection (ATCC). Molm-13 and EOL-1 cell lines were cultured in RPMI with 10% heat-inactivated FBS (Sigma-Aldrich), and MV4-11 in IMDM with 10% FBS. All cells were incubated at 37°C in a 5% CO2 humidified incubator.
Lu T., Chen L., Mansour A.G., Yu M.J., Brooks N., Teng K.Y., Li Z., Zhang J., Barr T., Yu J, & Caligiuri M.A. (2021). Cbl-b is upregulated and plays a negative role in activated human NK cells. Journal of immunology (Baltimore, Md. : 1950), 206(4), 677-685.
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5

Culturing Human Acute Myeloid Leukemia Cell Lines

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The MOLM-13 and EOL-1 human acute myeloid leukemia cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and they were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA), supplemented with FBS (10%). Cells were incubated at 37 °C in 5% CO2 and maintained with penicillin (100 U/mL) and streptomycin (100 μg/mL).
Ren Y., Gallucci J.C., Li X., Chen L., Yu J, & Kinghorn A.D. (2018). Crystal Structures and Human Leukemia Cell Apoptosis Inducible Activities of Parthenolide Analogues Isolated from Piptocoma rufescens. Journal of natural products, 81(3), 554-561.
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