Acetic acid (reagent grade, ≥99%), diethyl ether, deionized water, methanol (puriss. p.a., absolute, ≥99.8% (GC)), p-toluenesulfonic acid monohydrate (ACS reagent, ≥98.5%), tert-butyl nitrite (90%), 4-nitroaniline (≥99%), and PEDOT:PSS water suspension was supplied from Sigma-Aldrich. 4-Nitrobenzenediazonium tosylate was synthesized according to the published procedure.29 (link)
Tert butyl nitrite
Manufactured by Merck Group
Sourced in United States
Tert-butyl nitrite is a colorless liquid chemical compound used in various laboratory applications. It is a nitro compound with the chemical formula (CH3)3CONO. The core function of tert-butyl nitrite is as a nitrosating agent, which can be used for certain chemical reactions and syntheses in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
4 nitroaniline, by Merck Group (3 mentions)
P toluenesulfonic acid monohydrate, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Diethyl ether, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Methanol, by Merck Group (1 mentions)
Toluene, by Thermo Fisher Scientific (1 mentions)
Sodium sulfate, by Thermo Fisher Scientific (1 mentions)
Sodium iodide, by Merck Group (1 mentions)
Anhydrous diethyl ether, by Thermo Fisher Scientific (1 mentions)
Hexane, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Anhydrous tetrahydrofuran thf, by Merck Group (1 mentions)
Copper 1 chloride, by Merck Group (1 mentions)
Bisphenol a, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Methyl iodide, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
11 protocols using tert butyl nitrite
1
Synthesis of 4-Nitrobenzenediazonium Tosylate
2
Bisphenol A Functionalization Protocols
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All reagents were used
without further purification unless otherwise mentioned. Bisphenol
A (99+%), boron trifluoride etherate, methyl iodide, anhydrous tetrahydrofuran
(THF), tert-butyl nitrite, sodium chloride, copper(I)
chloride, potassium carbonate (K2CO3, 99+%),
benzyl chloride, TMHD, sodium iodide, cesium carbonate, and anhydrous
NMP 99% were purchased from Sigma-Aldrich Chemical Co. and used as
received. Hexane, anhydrous diethyl ether, anhydrous sodium thiosulfate,
methylene chloride stabilized, sodium hydroxide (97%), toluene, methanol,
ethyl acetate, and 37% hydrochloric acid were purchased from Fisher
Chemicals and used without further modification. Acetonitrile was
purchased from Spectrum and used without further modification. Sodium
sulfate was purchased from Alfa Aesar and used without further modification.
All solvents were dried and purified by using the standard procedures.
without further purification unless otherwise mentioned. Bisphenol
A (99+%), boron trifluoride etherate, methyl iodide, anhydrous tetrahydrofuran
(THF), tert-butyl nitrite, sodium chloride, copper(I)
chloride, potassium carbonate (K2CO3, 99+%),
benzyl chloride, TMHD, sodium iodide, cesium carbonate, and anhydrous
NMP 99% were purchased from Sigma-Aldrich Chemical Co. and used as
received. Hexane, anhydrous diethyl ether, anhydrous sodium thiosulfate,
methylene chloride stabilized, sodium hydroxide (97%), toluene, methanol,
ethyl acetate, and 37% hydrochloric acid were purchased from Fisher
Chemicals and used without further modification. Acetonitrile was
purchased from Spectrum and used without further modification. Sodium
sulfate was purchased from Alfa Aesar and used without further modification.
All solvents were dried and purified by using the standard procedures.
3
Synthesis of Arenediazonium Tosylates
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Chloroauric acid tetrahydrate (HAuCl4⋅ 4 H2O, 99.9 %), silver nitrate (AgNO3, 99.0 %), ascorbic acid (AA, 99.0 %), p‐toluenesulfonic acid monohydrate (98.0 %), acetic acid (≥99.7 %), tert‐butyl nitrite (90.0 %), 4‐nitroaniline (≥99 %), 4‐aminobenzoic acid (≥99 %), 4‐aminoaniline (≥99.0 %), and diethyl ether (≥99.7 %) were purchased from Sigma–Aldrich. All chemical reagents were used as received without further purification. Mueller–Hinton agar (MHA) was prepared as described by the producer (Oxoid, CM0337) and sterilized in an autoclave. Deionized water was used throughout the experiments. The corresponding arenediazonium tosylates (4‐nitrobenzenediazonium tosylate, 4‐aminobenzenediazonium tosylate, and 4‐carboxybenzenediazonium tosylate) were prepared according to the described procedure.55
4
Dendrimer-based Photoactive System
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Poly(amidoamine) dendrimer (generation 4.0, G4‐NH2, water solution, 10% wt/wt) was purchased from Dendritech. tert‐Butyl nitrite (TBN) and fluorescein 5(6)‐isothiocyanate (FITC, ≥90%) was obtained from Sigma‐Aldrich. PVP (average Mw = 40 kDa), N‐acetyl‐d ‐penicillamine (NAP), and chlorin e6 (Ce6) were purchased from Aladdin. All other chemicals and solvents were used without further purification unless mentioned. Cell Counting Kit‐8 (CCK‐8) was obtained from Dojindo Molecular Technologies. Polydimethylsiloxane (PDMS) female molds (height: ~1000 μm, base width: ~500 μm, spacing: ~1000 μm, 5 × 5 array) were purchased from the Research Institute of Zhejiang University‐Taizhou. Optimum cutting temperature (OCT) compounds were provided by Sakura Finetek.
5
Oxidative Functionalization of Cyclic Ketones
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The following were used: cyclopentanone (Sigma-Aldrich, St. Louis, MI, USA, ≥99%), cyclohexanone (Sigma-Aldrich, ≥99%), cycloheptanone (Sigma-Aldrich, ≥99%), cyclooctanone (Sigma-Aldrich, ≥98%), cyclododecanone (Merck, Rahway, NJ USA, ≥98%), 2-methylcyclohexanone (Aldrich, St. Louis, MI, USA, ≥98%), 3-methylcyclohexanone (Aldrich, ≥97%), 4-methylcyclohexanone (Aldrich, ≥99%), cyclohexanol (Sigma-Aldrich, >99%), cyclohexane (Sigma-Aldrich, >99%), manganese(II) acetylacetonate (Sigma-Aldrich, 99%), cobalt(II) acetylacetonate (Sigma-Aldrich, 97%), manganese(II) acetate tetrahydrate (Sigma-Aldrich, 99.99%), cobalt(II) acetate tetrahydrate (Sigma-Aldrich, 99.99%), manganase(II) nitrate tetrahydrate (Sigma-Aldrich, ≥97%), cobalt(II) nitrate hexahydrate (Sigma-Aldrich, >99%), isopentyl nitrite (Sigma-Aldrich, 96%), tert-butyl nitrite (Sigma-Aldrich, 90%), N-hydroxyphthalimide (Acros, Waltham, MA, USA, 98%), methyl ethyl ketone (Sigma-Aldrich, 99.7%), nitric acid (Sigma-Aldrich, 60%), pentyl nitrite (Sigma-Aldrich, 98%), acetic acid (Chempur, Karlsruhe, Germany, 99.5%), acetonitrile (Chempur, 99.5%), benzonitrile (Sigma-Aldrich, 99%), sulfuric acid (Chempur, 98%), and methanol (Chempur, 99.8%).
6
Synthesis and Characterization of N-Acetyl-D-penicillamine Functionalized PDMS
7
Antibacterial Gelatin-PCL Hydrogel Synthesis
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Poly(ε-caprolactone)
(PCL) (80 kDa) and Type A gelatin from porcine skin, alginate lyase,
anhydrous copper(II) sulfate,l -cysteine, l -cystine,
and tert-butylnitrite were purchased from Sigma-Aldrich
(St. Louis, MO, USA). Glutaraldehyde (25% EtOH) solution was purchased
from Thermo Fisher Scientific Inc., Waltham, MA, USA. Hexafluoroisopropanol
(HFIP) was purchased from Oakwood Chemicals (Estill, SC, USA). Gibco
(Thermo Fisher Scientific Inc., Waltham, MA, USA) supplied the fetal
bovine serum (FBS), minimum necessary medium (MEM), and penicillin–streptomycin.
The bacterial Live/Dead assay kit and Greiss assay kit were purchased
from Thermo Fisher Scientific Inc., Waltham, MA, USA. Cell viability
CCK-8 assay kit was obtained from Dojindo Laboratories, Rockville,
MD, USA. Methicillin-resistant S. aureus (MRSA) USA300 LAC was obtained from the University of Nebraska Medical
Center (UNMC). Luria–Bertani (LB) bacterial medium was purchased
from Thermo Fisher Scientific Oxoid (Waltham, MA, USA).
(PCL) (80 kDa) and Type A gelatin from porcine skin, alginate lyase,
anhydrous copper(II) sulfate,
and tert-butylnitrite were purchased from Sigma-Aldrich
(St. Louis, MO, USA). Glutaraldehyde (25% EtOH) solution was purchased
from Thermo Fisher Scientific Inc., Waltham, MA, USA. Hexafluoroisopropanol
(HFIP) was purchased from Oakwood Chemicals (Estill, SC, USA). Gibco
(Thermo Fisher Scientific Inc., Waltham, MA, USA) supplied the fetal
bovine serum (FBS), minimum necessary medium (MEM), and penicillin–streptomycin.
The bacterial Live/Dead assay kit and Greiss assay kit were purchased
from Thermo Fisher Scientific Inc., Waltham, MA, USA. Cell viability
CCK-8 assay kit was obtained from Dojindo Laboratories, Rockville,
MD, USA. Methicillin-resistant S. aureus (MRSA) USA300 LAC was obtained from the University of Nebraska Medical
Center (UNMC). Luria–Bertani (LB) bacterial medium was purchased
from Thermo Fisher Scientific Oxoid (Waltham, MA, USA).
8
Synthesis of Gold Nanoparticles on Zeolites
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Beta and MFI (CBV2314) zeolites were purchased
from Zeolyst International. Hydrogen tetrachloroaurate(III) hydrate
(99.9%) and 4-aminothiophenol (97.0%) were purchased from Alfa-Aesar.
Tetrafluoroboric acid solution (48.0 wt % in H2O), tert-butyl nitrite (90.0%), silicon dioxide (0.85–4.75
mm, 99.9%), and 3,5-di-tert-butyl-4-hydroxybenzylalcohol
were purchased from Sigma-Aldrich. Benzyl alcohol (99.0%) and dodecane
(99.0%) were purchased from Tokyo Chemical Industry. Acetonitrile
(99.5%), diethyl ether (99.5%), acetone (99.5%), and chloroform (99.8%)
were purchased from Samchun. All reagents were used as received.
from Zeolyst International. Hydrogen tetrachloroaurate(III) hydrate
(99.9%) and 4-aminothiophenol (97.0%) were purchased from Alfa-Aesar.
Tetrafluoroboric acid solution (48.0 wt % in H2O), tert-butyl nitrite (90.0%), silicon dioxide (0.85–4.75
mm, 99.9%), and 3,5-di-tert-butyl-4-hydroxybenzylalcohol
were purchased from Sigma-Aldrich. Benzyl alcohol (99.0%) and dodecane
(99.0%) were purchased from Tokyo Chemical Industry. Acetonitrile
(99.5%), diethyl ether (99.5%), acetone (99.5%), and chloroform (99.8%)
were purchased from Samchun. All reagents were used as received.
9
Purification and Radical Polymerization Protocol
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All chemicals were purchased from Sigma-Aldrich (Overijse, Belgium), Acros (Geel, Belgium) and TCI (Zwijndrecht, Belgium). 2,2,6,6-Tetramethyl-1-piperidyl methacrylate (TMPM, 98%, TCI), oligo(ethylene glycol) methyl ether methacrylate (OEGMA, average molar mass of 300 g/mol, Sigma-Aldrich) and di(ethylene glycol) dimethacrylate (OEGMA2, 98%, Sigma-Aldrich) were purified on a AlOx-filtration column prior use in order to remove the inhibitor. The 2,2′-azobisisobutyronitrile (AIBN, 98% purity, Sigma Aldrich) initiator was recrystallized twice from methanol (Acros, 99%) prior use. Acetylsalicylic acid (ASA, 99.9%, Sigma-Aldrich), isopropanol (IPA, Acros, 99.5%), methanol (Acros, 99.8%), diethyl ether (Acros, 99%), NaHCO3 (Sigma Aldrich, 99%), Na2WO4.2H2O (Sigma-Aldrich, 99%), H2O2 (Sigma-Aldrich, 30 wt% solution in water), ethylene diamine tetraacetic acid disodium salt dihydrate (EDTA, Sigma Aldrich, 99%), NaClO (Sigma Aldrich, 99%), HBF4 (Sigma Aldrich, 48 wt% solution in water), NaClO4 (Sigma Aldrich, 98%), acetonitrile (ACN, 99.9%, Sigma Aldrich), formic acid (98%, Sigma Aldrich), benzylic alcohol (Sigma Aldrich, 99%) and tert-butyl nitrite (Sigma Aldrich, 90%) were used as received.
10
Synthesis of Aryl Diazonium Salts
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Aryl diazonium salt D-NO 2 was prepared by the method published previously by our group. 35 2 g of 4-nitroaniline (≥99%, Sigma-Aldrich) and 10 mL of acetonitrile (≥99.9%, Sigma-Aldrich) were sequentially added to 12 mL of tetrafluoroboric acid (48% in H 2 O, Sigma-Aldrich). Then the mixed solution was transferred to an ice water bath and cooled down to 2 °C under continuous stirring. Afterwards, 2 mL of tert-butyl nitrite (90%, Sigma-Aldrich) was added to the reaction solution drop by drop. The solution was incubated for 30 min under stirring. After 3 times washes by diethyl ether (≥99.7%, Sigma-Aldrich), the resulting diazonium salt D-NO 2 was dissolved in acetone (≥99.9%, Sigma-Aldrich) and kept at room tempera-ture for drying. For the preparation of D-CCH and D-CN, 4-ethynylaniline (97%, Sigma-Aldrich) and 4-aminobenzonitrile (98%, Sigma-Aldrich) were used as corresponding precursors based on the same protocol.
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