Amyloid β1 42

Manufactured by Merck Group

Sourced in United States

Amyloid-β1–42 is a synthetic peptide that corresponds to the 1-42 amino acid sequence of the amyloid-beta protein. It is commonly used in research related to Alzheimer's disease and other neurodegenerative disorders.

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Lab products found in correlation

Coumarin 6, by Merck Group (1 mentions) Neuro2a cell line, by American Type Culture Collection (1 mentions) Curcumin, by Meilun (1 mentions) Hmgb1, by Merck Group (1 mentions)

Close spelling variants of this product

Amyloid β1–42 peptide Amyloid-β1–42 (Aβ1–42)

3 protocols using amyloid β1 42

Intranasal Nanoparticle Delivery for Alzheimer's

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Tf, amyloid-β_1–42, and 6-coumarin were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Curcumin (purity 99.5%) was purchased from Dalian Meilun Biology Technology Ltd. (Dalian, People’s Republic of China). D,L-Lactide and glycolide were obtained from PURAC (Gorinchem, the Netherlands). Maleimide-PEG (molecular weight [MW] 3,500 Da) was purchased from Jenkem Technology Corporation (Beijing, People’s Republic of China). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) dye was purchased from AAT Bioquest Inc. (Sunnyvale, CA, USA). All the other chemicals were of analytical reagent grade and used without further purification. BALB/c mice (male, weighing 20–22 g), nude mice (male, weighing 18–20 g), and C57BL/6 mice (male, weighing 20–22 g) were obtained from Shanghai SLAC Laboratory Animal Co. Ltd. (Shanghai, People’s Republic of China). The Neuro-2a cell line was obtained from ATCC (Manassas, VA, USA). The mouse brain capillary endothelial cell line (bEnd.3) was a gift from Professor Yaocheng Rui (Department of Pharmacology, The Second Military Medical University, People’s Republic of China).
All animal experiments were approved by the Ethical Committee of the Second Military Medical University. Animals were housed and fed according to the guidelines set by the Second Military University.

Jia T., Sun Z., Lu Y., Gao J., Zou H., Xie F., Zhang G., Xu H., Sun D., Yu Y, & Zhong Y. (2016). A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β1–42-injected mice. International Journal of Nanomedicine, 11, 3765-3775.

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Neuroprotective Effects of Anthocyanin Nanoparticles

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Amyloid-β_1–42 (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sterile saline at concentration of 1 mg/ml to prepare stock solution. This solution was incubated at 37 °C for 4 days. The SH-SY5Y cells (purchased from Korean Cell Bank, South Korea) were maintained in a solution DMEM, 10% fetal bovine serum (FBS) and antibiotics (penicillin and streptomycin), grown for 5 days and treated as follows: (1) Control: incubated in DMEM solution for 24 h; (2) Aβ_1–42-treatment: incubated in DMEM solution containing Aβ_1–42 (5 µM) for 24 h; (3) Aβ_1–42 + native anthocyanin treatment: incubated in DMEM solution containing Aβ_1–42 (5 µM) for 12 h and then post incubated with native anthocyanin (200 µg/ml) for 12 h; (4) Aβ_1–42 + An-NPs treatment: incubated in DMEM solution containing Aβ_1–42 (5 µM) for 12 h and then post incubated with An-NPs (200 µg/ml) for 12 h; All the cells were harvested at day 6 and used for the desired analysis.

Amin F.U., Shah S.A., Badshah H., Khan M, & Kim M.O. (2017). Anthocyanins encapsulated by PLGA@PEG nanoparticles potentially improved its free radical scavenging capabilities via p38/JNK pathway against Aβ1–42-induced oxidative stress. Journal of Nanobiotechnology, 15, 12.

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RAGE Binding Assay with Oxytocin

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Oxytocin binding to RAGE and competition by RAGE ligands were assayed with oxytocin-coated 96-well plates, human esRAGE, and horseradish peroxidase (HRP)-conjugated anti-human RAGE antibody^{27 (link)}. Oxytocin (100 μl, indicated concentrations) was immobilised in 96-well microtitre plates, 1 μg/ml esRAGE was added, and bound esRAGE was detected immunochemically using covalently coupled 〈RAGE antibody- horseradish peroxidise-catalysed oxidation of tetramethylbenzidine. RAGE ligands were S100B (Sigma-Aldrich, St. Louis, MO, USA)^{30 (link)}, AGE-BSA (glyceraldehyde-derived AGE-BSA)^{27 (link),32 (link),41 (link)}, amyloid β 1-42 (Sigma-Aldrich), and high-mobility group box 1 (HMGB1, Sigma-Aldrich)^{42 (link)}.

Yamamoto Y., Liang M., Munesue S., Deguchi K., Harashima A., Furuhara K., Yuhi T., Zhong J., Akther S., Goto H., Eguchi Y., Kitao Y., Hori O., Shiraishi Y., Ozaki N., Shimizu Y., Kamide T., Yoshikawa A., Hayashi Y., Nakada M., Lopatina O., Gerasimenko M., Komleva Y., Malinovskaya N., Salmina A.B., Asano M., Nishimori K., Shoelson S.E., Yamamoto H, & Higashida H. (2019). Vascular RAGE transports oxytocin into the brain to elicit its maternal bonding behaviour in mice. Communications Biology, 2, 76.

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