U46619

Collagen (Kollagenreagent Horm; Nycomed), convulxin (Enzo Lifesciences) α-thrombin (Roche Diagnostics), adenosine diphosphate (ADP), sodium heparin, human fibrinogen, apyrase type III, prostacyclin (PGI₂), Igepal CA-630 (all from Sigma-Aldrich), U46619 (Alexis Biochemicals) and ECL solution (PerkinElmer) were purchased, collagen-related peptide (CRP) was generated as described [21] (link). Rhodocytin was a generous gift from Prof. Dr. J. Eble (Münster University Hospital, Germany). The anesthetic drugs medetomidine (Pfizer), midazolam (Roche Pharma AG), and fentanyl (Janssen-Cilag GmbH) and the antagonists atipamezol (Pfizer), flumazenil, and naloxon (both from Delta Select GmbH) were used according to the regulation of the local authorities. The antibody against the activated form of integrin αIIbβ3 (JON/A-PE) was from Emfret Analytics. Anti-murine CD84 monoclonal antibody JER1 [19] (link) and other antibodies were generated and modified in our laboratories as described [22] (link).

Thrombin (Roche Diagnostics), adenosine diphosphate (ADP, Sigma-Aldrich), high-molecular-weight heparin (Sigma-Aldrich), human fibrinogen (Sigma-Aldrich), U46619 (Alexis Biochemicals), collagen (Horm Kollagen, Nycomed), apyrase (Amersham/GE Healthcare), thapsigargin (TG, Invitrogen), Fura-2/AM (Invitrogen), and Pluronic F-127 (Molecular Probes), IP₁ ELISA kit (Cisbio Bioassays) were purchased. Monoclonal antibodies conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), or DyLight-488 and the antibody against the activated form of integrin αIIbβ3 (JON/A-PE) were from Emfret Analytics (Eibelstadt, Germany). Collagen-related peptide (CRP) was generated as described [35 (link)].