H cys arg arg arg nh2

Reagents and solvents used for polymer synthesis were purchased from Sigma-Aldrich and Panreac. Oligopeptide moieties used on the polymer modification (H-Cys-Arg-Arg-Arg-NH2, H-Cys-Lys-Lys-Lys-NH2, H-Cys-His-His-His-NH2, and H-Cys-Asp-Asp-Asp-NH2) were obtained from GL Biochem (Shanghai) Ltd. with a purity of at least 98%. Labeled siRNA (AllStars Neg. siRNA AF 555) for uptake experiments was purchased from Qiagen. ON-TARGETplus Non-Targeting Control Pool (D-001810-10) used as siRNA control was obtained from Thermo GE Dharmacon. Polyplus Interferin reagent was purchased from VWR and used according to manufacturer instructions. siRNA against ICAM2 (termed siICAM2) with forward sequence 5′-AGGACGGUCUCAACUUUUC-3′ and reverse sequence 5′-GAAAAGUUGAGACCGUCCU-3′ and siRNA against luciferase (used as a negative control and termed siCON) were used, all obtained from AXOlabs. RNA extraction was carried out using the Direct-zol RNA kit from Genesee Scientific, cDNA reaction was performed using the Applied Biosystems cDNA kit, and cDNA quantification was analyzed using SYBR green from Affymetrix, following the manufacturer’s instructions.

Reagents and solvents used for polymer synthesis were purchased from Sigma-Aldrich and Panreac. Oligopeptide moieties used for polymer modification (H-Cys-Arg-Arg-Arg-NH₂, H-Cys-Lys-Lys-Lys-NH₂, H-Cys-His-His-His-NH₂, and H-Cys-Asp-Asp-Asp-NH₂) were obtained from GL Biochem (Shanghai) Ltd. with a purity of at least 98%. VHPK peptide (VHPKQHRGGSKGC) was obtained from GenScript with a purity of at least 98%. Labeled anti-miR (Cy3™ Dye-Labeled Anti-miR™ Negative Control #1; AM17011) and Anti-miR™ miRNA Inhibitor Negative Control #1 (AM17010) were used for uptake experiments and purchased from ThermoFisher. Anti-miR-712 and scramble anti-miR (anti-miR-SCR) control were purchased from Qiagen. Oligofectamine™ Transfection Reagent was purchased from Invitrogen and used according to manufacturer instructions.