Uplansapo 60 oil immersion objective

Manufactured by Olympus

Sourced in Japan

The UPlanSApo 60× oil immersion objective is a high-performance microscope lens designed for advanced microscopy applications. It features a numerical aperture of 1.35 and a working distance of 0.15 mm, providing high-resolution imaging capabilities. The objective is optimized for use with oil immersion, which helps to improve image quality and contrast. Its core function is to serve as a precision optical component in research-grade microscopes, enabling detailed observation and analysis of samples.

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Lab products found in correlation

Fv1000 confocal microscope, by Olympus (2 mentions) Lsm 980 confocal microscope, by Zeiss (1 mentions) Plan apochromat 63x, by Zeiss (1 mentions) Fv1000, by Olympus (1 mentions) Uplansapo 100 oil immersion objective, by Olympus (1 mentions) Calcofluor white m2r, by Merck Group (1 mentions)

5 protocols using uplansapo 60 oil immersion objective

Multi-modal Superresolution Imaging of Organelles

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Superresolution images were acquired in a Zeiss LSM980 confocal microscope (Carl Zeiss) equipped with the AiryScan 2 detector using a Plan-Apochromat 63x oil immersion objective (NA = 1.4). EGFP-XTP and MitoTracker Deep Red FM were excited with solid diode lasers of 488 nm and 639 nm, respectively. Images were acquired sequentially by frame with pixel size of

\sim

40 nm. Raw files were deconvoluted using the standard processing routine from the Zen software.
Confocal images were acquired in FV1000 Olympus confocal microscope (Olympus Inc). EGFP-XTP and MitoTracker Deep Red FM were observed using a multi-line Ar laser tuned at 488 nm and a 635 nm solid diode laser as excitation sources, respectively. The laser’s light was reflected by a dichroic mirror (DM405/488/543/635) and focused through an Olympus UPlanSApo 60× oil immersion objective (NA = 1.35) onto the sample. Fluorescence was collected by the same objective and split into two channels set to collect photons in the range 505–525 nm (EGFP) and 655–755 nm (MitoTracker Deep Red FM). Fluorescence was detected with photomultipliers set in the photon-counting detection mode. Time-lapse images were acquired at a speed of (0.1–1.96 frames/s). Pixel size: 0.055–0.276

μ

Fernández Casafuz A.B., De Rossi M.C, & Bruno L. (2023). Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks. Scientific Reports, 13, 4065.

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Visualizing Filopodia in PC3 Cells

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PC3 cells (transfected or otherwise) were fixed with 8% paraformaldehyde (20 min, room temperature) and stained with rhodamine–phalloidin (1 h, room temperature). Confocal images were acquired by confocal microscopy (FV1000, Olympus, Tokyo, Japan) using an UPlanSApo 60× oil immersion objective (NA 1/41.35; Olympus), a diode laser of 543 nm as the excitation source and fluorescence was collected in the range of 555–655 nm. We selected the regions closest to the substrate from which filopodia were clearly defined.
Confocal microscope images were analyzed using ImageJ software (NIH, Bethesda, MD, USA). Cells were considered positive (high-filopodia count) if they presented more than three or more filopodia sets.

Dalton G.N., Massillo C., Scalise G.D., Duca R., Porretti J., Farré P.L., Gardner K., Paez A., Gueron G., De Luca P, & De Siervi A. (2019). CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice. Cell Death & Disease, 10(4), 299.

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Imaging Mitochondrial Dynamics in Cells

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Confocal images were acquired in an FV1000 confocal microscope (Olympus Inc.). EGFP-XTP and MitoTracker Deep Red FM were observed using a multi-line Ar laser tuned at 488 nm and a 635-nm diode laser as excitation sources (average power at the sample, 2 and 0.2 μW, respectively). The laser’s light was reflected by a dichroic mirror (DM 405/488/543/635) and focussed through an Olympus UPlanSApo 60× oil immersion objective (NA = 1.35) on to the sample. Fluorescence was collected by the same objective and split into two channels set to collect photons in the range 500–525 nm (EGFP) and 650–750 nm (MitoTracker Deep Red FM). Fluorescence was detected with photomultipliers set in the photon-counting detection mode.
Time-lapse confocal movies (100–150 frames, pixel size = 63 nm) of individual fluorescent mitochondria were collected at a speed of 0.6 frames/s.

De Rossi M.C., Levi V, & Bruno L. (2018). Retraction of rod-like mitochondria during microtubule-dependent transport. Bioscience Reports, 38(3), BSR20180208.

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Confocal Microscopy for Cell Imaging

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Imaging was performed using confocal microscopy (Olympus Fluoview FV3000, IX83) and a UPlanSApo 40× siliconeimmersion objective (numerical aperture [NA], 1.25), UPlanSApo 60× oil-immersion objective (NA, 1.40), or UPlanSApo 100× oil-immersion objective (NA, 1.40). Images were acquired with Fluoview FV31S-SW software, and imaging analysis was completed in FIJI. 26 (link) For each replicate within an experiment, images were acquired at the same settings.

Tanke N.T., Liu Z., Gore M.T., Bougaran P., Linares M.B., Marvin A., Sharma A., Oatley M., Yu T., Quigley K., Vest S., Cook J.G, & Bautch V.L. (2024). Endothelial Cell Flow-Mediated Quiescence Is Temporally Regulated and Utilizes the Cell Cycle Inhibitor p27. Arteriosclerosis, thrombosis, and vascular biology, 44(6).

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Quantitative Analysis of Root Cell Walls

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Apical portions (approximately 1 cm) of C and S roots (ten roots of each of the two groups) were stained with 0.01% Calcofluor White M2R (Sigma, F3543, Darmstadt, Germany) in phosphate-buffered saline (PBS, pH 7.2) for 15 min in darkness and washed three times with deionized water. Next, the roots were split centrally along their main axes and the halves were placed in a water chamber between two coverslips separated by a silicone spacer frame (Press-to-Seal silicone sheet with adhesive, ThermoFisher Scientific). Images of the two layers of pellicle (L3, L2) and of the outer protodermal cell wall (L1) were acquired in a plane tangential to the root surface (see Figure 3) using an Olympus FluoView FV1000 confocal laser scanning microscope with a UPlanSApo 60× oil immersion objective (NA 1.35). Calcofluor White was excited with a 405 nm laser diode and emission was collected between 425 and 525 nm. Z-stacks of optical sections (image size 1024 × 1024 pixels, pixel size 0.103 μm) were taken with an interval of 0.13 μm. Image processing, including orthogonal projections and 3D reconstructions of z-stacks, was performed applying ImageJ software.
Application details of the used staining techniques are specified in Table A1 (Appendix B).

Potocka I, & Szymanowska-Pułka J. (2021). The Pellicle–Another Strategy of the Root Apex Protection against Mechanical Stress?. International Journal of Molecular Sciences, 22(23), 12711.

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