Split peas were purchased from Foshan Jinnuoyi Processing Factory of Agricultural Products (Foshan, China); the protein content was 21.12% (6.25 of N, wet weight). Grass carp were purchased from the local fish market in Zhanjiang City, Guangdong Province. The gills, heads, tails, bones, and blood were removed, leaving only the white muscles on the back, which were retained and frozen at a low temperature. The fish muscles were brought back to the laboratory and stored at −20 °C; the protein content was 18.27% (6.25 of N, wet weight). Electrophoresis reagents were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The ANS fluorescence probe was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Other reagents were of analytical grade and were ordered from the Guangzhou Chemical Reagent Factory (Guangzhou, China).
Electrophoresis reagents
Manufactured by Beyotime
Sourced in China
Electrophoresis reagents are a collection of chemical solutions and buffers used in the process of electrophoresis. Electrophoresis is a laboratory technique used to separate and analyze molecules, such as proteins or nucleic acids, based on their size and electrical charge. The reagents provide the necessary components for this separation process.
Automatically generated - may contain errors
Lab products found in correlation
L rhamnose, by Merck Group (1 mentions)
L rhamnulose, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Beyotime (1 mentions)
Genomic dna mini preparation kit with a spin column, by Beyotime (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Universal dna purification kit, by Tiangen Biotech (1 mentions)
Dna gel extraction kit, by Generay (1 mentions)
2 protocols using electrophoresis reagents
1
Extraction and Characterization of Pea and Fish Proteins
2
Chemicals and Reagents for Molecular Biology
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Standard substances such as L-rhamnulose and L-rhamnose were purchased from Sigma-Aldrich (USA). Electrophoresis reagents, the genomic DNA minipreparation kit with a spin column, the polymerase chain reaction (PCR) kit, and isopropyl β-D-1thiogalactopyranoside (IPTG) were purchased from Beyotime Institute of Biotechnology (China). Restriction enzymes and T4 DNA ligase were purchased from New England Biolabs (USA). A DNA gel extraction kit was purchased from Generay Biotech (China). A universal DNA purification kit was obtained from Tiangen Biotech Co., Ltd. (China). All other reagents (analytical grade) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China).
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