Ransel rs505

Manufactured by Randox

Sourced in United Kingdom

The RANSEL RS505 is a laboratory instrument designed for the detection and quantification of a wide range of analytes in various sample types. It utilizes advanced analytical techniques to provide accurate and reliable results. The core function of the RANSEL RS505 is to serve as a versatile diagnostic tool for research and clinical applications.

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Lab products found in correlation

Ransod sd125, by Randox (3 mentions) Versamax tunable microplate reader, by Molecular Devices (2 mentions) Tbars assay kit, by Cayman Chemical (2 mentions) Bs 240 vet clinical chemistry analyser, by Mindray (1 mentions) Nx 2332, by Randox (1 mentions)

5 protocols using ransel rs505

Lipid Peroxidation and Antioxidant Enzyme Evaluation

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MDA levels in tissues were evaluated using commercially available assay kits to determine the lipid peroxidation state (TBARS Assay Kit, item no. 10009055, Cayman Chemicals, Ann Arbor, MI, USA). The measuring principle is based on the reaction with thiobarbituric acid (TBA) in boiling water for 60 min in an acidic medium and the measurement of the absorbance of the reaction mixture at 532 nm (Ohkawa et al., 1979). A VersaMax Tunable Microplate Reader was used to measure the absorbance (Molecular Devices, San Jose, CA, USA). MDA concentrations in tissues were expressed as nmol MDA/mg protein.
In tissues, GPx and SOD activity were evaluated using ready-to-use assay kits. (RANSEL RS505, RANSOD SD125, Randox Laboratories Ltd., Crumlin, UK) by using an automated BS-240 VET Clinical Chemistry Analyser (Mindray, Shenzhen, China). The quantities of GPx and SOD in the tissue samples (stomach, small intestine, and large intestine) were expressed as U/mg protein.

Ceylanlı D., Şehirli A.Ö., Gençosman S., Teralı K., Şah H., Gülmez N, & Sayıner S. (2022). Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats. Antioxidants, 11(10), 1930.

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Antioxidant Status and Enzyme Activity

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Plasma total antioxidant status (TAS) was measured with the use of a kit test (NX 2332, produced by Randox Laboratories, Antrim, UK). The activity of superoxide dismutase (ESOD) and glutathione peroxide (GPx) in erythrocytes was determined using RANSOD SD125 and RANSEL RS505 tests (Randox Laboratories, Antrim, UK), respectively. The activity of ESOD and GPx were expressed as units per gram of hemoglobin (U/g Hb).

Daragó A., Sapota A., Nasiadek M., Klimczak M, & Kilanowicz A. (2016). The Effect of Zinc and Selenium Supplementation Mode on Their Bioavailability in the Rat Prostate. Should Administration Be Joint or Separate?. Nutrients, 8(10), 601.

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Oxidative Stress Biomarker Measurement

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The measured prooxidation markers were TBARS and PCs. Serum TBARS and PCs were measured with a spectrophotometric method as previously described (4). The intra-and interassay coefficients of variation (CV) were 4.3 and 6.8%, for TBARS, and 4.2 and 6.3% for PCs, respectively. The measured antioxidation markers were GSH and GSSG, the enzymes GPX and catalase as well as TAC. Erythrocyte lysate GSH and GSSG were measured with a spectrophotometric method as previously described (3). Serum TAC was measured with a spectrophotometric method as previously described (4).Erythrocyte lysate catalase activity was measured with a spectrophotometric method as previously described (3). Whole blood GPX was measured with a spectrophotometric method, with the use of a commercially available kit (Ransel RS 505; Randox, Crumlin, UK), as previously described (2) . The intra-and interassay CV were 3.7 and 6.4% for GSH, 5.5 and 7.6% for GSSG, 3.8 and 5.4% for TAC, 5.9 and 7.9% for catalase activity, and 4.5 and 4.4% for GPX, respectively.

Paltoglou G., Fatouros I.G., Valsamakis G., Schoina M., Avloniti A., Chatzinikolaou A., Kambas A., Draganidis D., Mantzou A., Papagianni M., Kanaka-Gantenbein C., Chrousos G.P, & Mastorakos G. (2015). Antioxidation improves in puberty in normal weight and obese boys, in positive association with exercise-stimulated growth hormone secretion. Pediatric research, 78(2).

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Antioxidant and Lipid Peroxidation Assay

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GPx and SOD activities were measured using ready-to-use assay kits in tissue homogenates (stomach, small intestine, liver, and kidney) (RANSEL RS505, RANSOD SD125, Randox Laboratories Ltd., Crumlin, County Antrim, UK) by using an automated BS-240 VET Clinical Chemistry Analyzer (Mindray, Shenzhen, China).
MDA concentrations were measured in tissue homogenates (stomach, small intestine, liver, and kidney) to assess lipid peroxidation status, using commercially available assay kits (TBARS Assay Kit, item no. 10009055, Cayman Chemicals, Ann Arbor, MI, USA). The principal measurement was based on the reaction with thiobarbituric acid (TBA) in boiling water for 60 min in an acidic medium, and measurement of the absorbance of the reaction mixture at 532 nm [28 (link)]. Absorbance was measured with VersaMax Tunable Microplate Reader (Molecular Devices, San Jose, CA, USA).

Gençosman S., Ceylanlı D., Şehirli A.Ö., Teralı K., Bölükbaşı F., Çetinel Ş, & Sayıner S. (2022). Investigation of the Possible Protective Effect of N-Acetylcysteine (NAC) against Irinotecan (CPT-11)-Induced Toxicity in Rats. Antioxidants, 11(11), 2219.

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Plasma Lipid and Oxidative Biomarker Analysis

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Biological samples were obtained after an overnight fast, coded, shipped to central laboratories, and frozen at −80°C until the assay. Plasma glucose and lipid analyses were performed in a PENTRA-400 autoanalyzer (ABX-Horiba Diagnostics, Montpellier, France). Soluble HDL cholesterol was measured by an accelerator selective detergent method (ABX-Horiba Diagnostics, Montpellier, France) and LDL cholesterol was calculated by the Friedewald equation whenever triglycerides were <3.4 mmol/L. Quality control was performed with UNITY External Quality Assessment (BIO-RAD, Hercules, CA, USA). Circulating oxidized LDL (ox-LDL) plasma levels were measured by a commercial enzyme-linked immunoabsorbent assay (Mercodia AB, Uppsala, Sweden). Intra- and inter-assay coefficients of variation were 2.8% and 7.3%, respectively. Plasma GSH-Px activity (GSH-Px; EC 1.11.1.9) was measured by a Paglia and Valentine [22] (link) modification method using cumene hydroperoxide (Ransel RS 505, Randox Laboratories, Crumlin, UK) as a glutathione oxidant. Intra- and inter-run imprecision were 3.6% and 5.43%, respectively.

Flores-Mateo G., Elosua R., Rodriguez-Blanco T., Basora-Gallisà J., Bulló M., Salas-Salvadó J., Martínez-González M.Á., Estruch R., Corella D., Fitó M., Fiol M., Arós F., Gómez-Gracia E., Subirana I., Lapetra J., Ruiz-Gutiérrez V., Sáez G.T, & Covas M.I. (2014). Oxidative Stress Is Associated with an Increased Antioxidant Defense in Elderly Subjects: A Multilevel Approach. PLoS ONE, 9(9), e105881.

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