Caakt

Manufactured by Addgene

Sourced in China

The CaAKT is a laboratory equipment designed for the measurement and analysis of calcium-activated kinase (AKT) activity. It provides researchers with a reliable tool to quantify the activation of this important signaling protein in various biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Jetprime, by Polyplus Transfection (1 mentions) Ha ubiquitin, by Addgene (1 mentions) Amaxa nucleofection system, by Lonza (1 mentions) Pcdna mdm2, by Addgene (1 mentions)

3 protocols using caakt

Activating AKT and SOX9 in Chondrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse primary chondrocytes and ATDC5 cells were transfected in monolayer using jetPRIME (Polyplus) according to the manufacturer's instructions. caAKT (Addgene plasmid 10841)23 (link) was used to constitutively activate AKT signalling, and a SOX9 plasmid was used to overexpress SOX9.24 (link)

Sherwood J., Bertrand J., Nalesso G., Poulet B., Pitsillides A., Brandolini L., Karystinou A., De Bari C., Luyten F.P., Pitzalis C., Pap T, & Dell'Accio F. (2014). A homeostatic function of CXCR2 signalling in articular cartilage. Annals of the Rheumatic Diseases, 74(12), 2207-2215.

+ Open protocol

+ Expand

Transfection and Protein Expression Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected using an Amaxa Nucleofection system (Amaxa Biosystems, Gaithersburg, MD) according to the manufacturer’s instructions. The plasmids encoding constitutively active (CA)-Akt, pcDNA-MDM2, and HA-ubiquitin were obtained from Addgene (Cambridge, MA), and Foxo3a shRNA from Origene (Rockville, MD). Foxo3a-GFP plasmid was kindly provided by Dr. Mickey C-T. Hu (Stanford University School of Medicine). Treatments were initiated 24 h after completion of 48-h transfection. Expression of various plasmids was confirmed by immunoblotting analysis.

Chou C.C., Lee K.H., Lai I.L., Wang D., Mo X., Kulp S.K., Shapiro C.L, & Chen C.S. (2014). AMPK Reverses the Mesenchymal Phenotype of Cancer Cells by Targeting the Akt-MDM2-Foxo3a Signaling Axis. Cancer research, 74(17), 4783-4795.

+ Open protocol

+ Expand

Plasmid Transfection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HA-HSP90 expression plasmid was kindly provided by Dr. Lu Zhang (Sichuan University, China). CA-Akt (myrAkt delta4-129) was purchased from Addgene (Chendu, China). The plasmids were introduced into cells using Lipofectamine 2000 according to the manufacturer's instructions.

Sun Y., Huang Y.H., Huang F.Y., Mei W.L., Liu Q., Wang C.C., Lin Y.Y., Huang C., Li Y.N., Dai H.F, & Tan G.H. (2018). 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Theranostics, 8(7), 2044-2060.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!