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Anti mitochondria clone 113 1

Manufactured by Merck Group
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Anti-mitochondria (clone 113-1) is a laboratory product manufactured by Merck Group. It is a monoclonal antibody that specifically binds to mitochondrial proteins. This product is intended for use in various research applications, such as the identification and analysis of mitochondrial structures and functions.

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Lab products found in correlation

Alexa fluor fluorescent dye conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions) Vimentin clone d21h3, by Cell Signaling Technology (2 mentions) Mouse cxcl12 sdf 1 alpha quantikine elisa kit, by R&D Systems (2 mentions) Anti pan cytokeratin clone c11, by Cell Signaling Technology (2 mentions) Anti α smooth muscle actin α sma clone 1a4, by Agilent Technologies (2 mentions) Anti cd105 pe clone sn6, by Thermo Fisher Scientific (2 mentions) Ccl5 duoset elisa kit, by R&D Systems (2 mentions) Anti ki67, by Abcam (2 mentions) Il 6 elisa kit, by BD (2 mentions) Annexin 5 pe apoptosis detection kit, by Thermo Fisher Scientific (1 mentions) F4 80 clone bm8, by Thermo Fisher Scientific (1 mentions) Mtt cell proliferation assay kit, by American Type Culture Collection (1 mentions)

3 protocols using anti mitochondria clone 113 1

1

Multiparametric Characterization of Cellular Phenotypes

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Antibodies used for staining included: anti-pan-cytokeratin (clone C11, 1:500) and vimentin (clone D21H3, 1:200) (Cell Signaling), anti- α-smooth muscle actin (α-SMA) (clone 1A4, 1:200) (Dako), anti-Ki-67 (rabbit polyclonal, ab15580, 1:300) (Abcam), anti-CD105-PE (clone SN6, 1:100) (eBioscience), anti-mitochondria (clone 113-1, 1:100) and anti-fibroblast specific protein-1 (FSP-1; rabbit polyclonal, 07-2274, 1:200) (Millipore). IL-6 ELISA kit was purchased from BD Biosciences. CCL5 DuoSet ELISA kit and mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit were from R&D Systems. Alexa Fluor® fluorescent dye-conjugated secondary antibodies were from Life Technologies for immunofluorescent staining.
Shen K., Luk S., Hicks D.F., Elman J.S., Bohr S., Iwamoto Y., Murray R., Pena K., Wang F., Seker E., Weissleder R., Yarmush M.L., Toner M., Sgroi D, & Parekkadan B. (2014). Resolving Cancer-Stroma Interfacial Signaling and Interventions with Micropatterned Tumor-Stromal Assays. Nature communications, 5, 5662.
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2

Multiparametric Analysis of Immune Cells

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Antibodies used for staining included: anti-CD19 (clone SJ25C1, 1:50, flow cytometry), F4/80 (clone BM8, 1:100, immunohistochemistry) (eBioscience), and anti-mitochondria (clone 113-1, 1:100) (Millipore). Annexin V PE apoptosis detection kit was purchased from eBioscience. Luminex xMAP multiplex kits for mouse immune cytokines were purchased from Millipore. MTT cell proliferation assay kit was purchased from ATCC. All assays were run following the manufacturers’ instructions.
Shen K., Luk S., Elman J., Murray R., Mukundan S, & Parekkadan B. (2016). Suicide Gene-Engineered Stromal Cells Reveal a Dynamic Regulation of Cancer Metastasis. Scientific Reports, 6, 21239.
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3

Multiparametric Characterization of Cellular Phenotypes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for staining included: anti-pan-cytokeratin (clone C11, 1:500) and vimentin (clone D21H3, 1:200) (Cell Signaling), anti- α-smooth muscle actin (α-SMA) (clone 1A4, 1:200) (Dako), anti-Ki-67 (rabbit polyclonal, ab15580, 1:300) (Abcam), anti-CD105-PE (clone SN6, 1:100) (eBioscience), anti-mitochondria (clone 113-1, 1:100) and anti-fibroblast specific protein-1 (FSP-1; rabbit polyclonal, 07-2274, 1:200) (Millipore). IL-6 ELISA kit was purchased from BD Biosciences. CCL5 DuoSet ELISA kit and mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit were from R&D Systems. Alexa Fluor® fluorescent dye-conjugated secondary antibodies were from Life Technologies for immunofluorescent staining.
Shen K., Luk S., Hicks D.F., Elman J.S., Bohr S., Iwamoto Y., Murray R., Pena K., Wang F., Seker E., Weissleder R., Yarmush M.L., Toner M., Sgroi D, & Parekkadan B. (2014). Resolving Cancer-Stroma Interfacial Signaling and Interventions with Micropatterned Tumor-Stromal Assays. Nature communications, 5, 5662.
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