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Cd10 bv786

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The CD10/BV786 is a laboratory instrument used for detecting and analyzing specific cell surface markers. It is a tool designed for researchers and scientists to study the expression of the CD10 antigen and other cell surface proteins.

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Lab products found in correlation

Cd45ra apc cy7, by BioLegend (2 mentions) Cd38 pe cy7, by BioLegend (1 mentions) Cd34 apc, by BD (1 mentions) Cd90 percp cy5, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions) Precision count beads, by BioLegend (1 mentions) Cd14 bv510, by BioLegend (1 mentions) Cd90 apc, by BD (1 mentions) Cd3 bv605, by BioLegend (1 mentions) Cd38 buv737, by BD (1 mentions) Cd11c bv650, by BD (1 mentions) Cd19 apc r700, by BD (1 mentions) Cd34 bv421, by BioLegend (1 mentions) Sphero rainbow calibration particles, by Spherotech (1 mentions) Cd135 pe, by BioLegend (1 mentions) Cd56 pe cy5, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Cd45 buv395, by BD (1 mentions)

2 protocols using cd10 bv786

1

Profiling COVID-19 Hematopoietic Stem and Progenitor Cells

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Purified BMMCs of the six COVID-19 patients were used to stain the HSPCs and flow cytometry was performed on FACSymphonyTM S6 (BD Biosciences). The following antibodies for the HSPCs were used: lineage cocktail (CD3, CD14, CD16, CD19, CD20, CD56/Pacific Blue) (Biolegend, clone UCHT1; HCD14, 3G8, HIB19, 2H7, HCD56, Cat. no. 348805, RRID: AB_2889063), CD34/APC (BD Biosciences, clone 581, Cat. no. 555824, RRID: AB_398614), CD38/PE-Cy7 (Biolegend, clone HIT2, Cat. no. 303516, RRID: AB_2072782), CD90/PerCP-Cy5.5 (BD Biosciences, clone 5E10, Cat. no. 561557, RRID: AB_10712762), CD45RA/APC-Cy7 (Biolegend, clone HI100, Cat. no. 304128, RRID: AB_10708880), CD49f/BV605 (BD Biosciences, clone GoH3, Cat. no. 740416, RRID: AB_2740146), CD10/BV786 (BD Biosciences, clone HI10a, Cat. no. 564960, RRID: AB_2739025), LIVE/DEAD dye/BV510 (Invitrogen, Cat. no. L34957), and Annexin V/FITC (BD Biosciences, Cat. no. 556547, RRID: AB_2869082). Gatings included HSC (Lin−CD34+CD38−CD45RA−CD90+CD49f+CD10−), MPP (Lin−CD34+CD38−CD45RA−CD90−CD10−).
Wang X., Wen Y., Xie X., Liu Y., Tan X., Cai Q., Zhang Y., Cheng L., Xu G., Zhang S., Wang H., Wei L., Tang X., Qi F., Zhao J., Yuan J., Liu L., Zhu P., Ginhoux F., Zhang S., Cheng T, & Zhang Z. (2021). Dysregulated hematopoiesis in bone marrow marks severe COVID-19. Cell Discovery, 7, 60.
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2

Comprehensive Bone Marrow Cell Immunophenotyping

Cited 1 time
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The detailed protocol for whole BM staining has been previously reported (Basso-Ricci et al., 2017 (link)). In brief, Precision Count beads (BioLegend) were added to 100 μl of P1 BM sample to allow absolute quantification of hematopoietic cell subsets, and red blood cell lysis was performed. The lysed sample was labeled with fluorescent antibodies for CD3-BV605, CD56-PE-Cy5, CD14-BV510, CD61/41-PE-Cy7, CD135-PE, CD34-BV421, CD45RA-APC-Cy7 (BioLegend), CD33-BB515, CD66b-BB515, CD38-BUV737, CD45-BUV395, CD90-APC, CD10-BV786, CD11c-BV650, CD19-APCR700, CD7-PE-Cy5.5, and CD71-BV711 (BD Biosciences). Titration assays were performed to assess the best antibody concentration. After surface marking, the cells were incubated with PI (BioLegend) to stain dead cells. All samples were acquired using a BD LSR Fortessa (BD Bioscience) flow cytometer after calibration with SPHERO rainbow calibration particles (Spherotech), and raw data were collected using BD FACSDIVA software. The data were subsequently analyzed with FlowJo software, and the graphical output was automatically generated using GraphPad Prism.
Lam M.T., Coppola S., Krumbach O.H., Prencipe G., Insalaco A., Cifaldi C., Brigida I., Zara E., Scala S., Di Cesare S., Martinelli S., Di Rocco M., Pascarella A., Niceta M., Pantaleoni F., Ciolfi A., Netter P., Carisey A.F., Diehl M., Akbarzadeh M., Conti F., Merli P., Pastore A., Levi Mortera S., Camerini S., Farina L., Buchholzer M., Pannone L., Cao T.N., Coban-Akdemir Z.H., Jhangiani S.N., Muzny D.M., Gibbs R.A., Basso-Ricci L., Chiriaco M., Dvorsky R., Putignani L., Carsetti R., Janning P., Stray-Pedersen A., Erichsen H.C., Horne A., Bryceson Y.T., Torralba-Raga L., Ramme K., Rosti V., Bracaglia C., Messia V., Palma P., Finocchi A., Locatelli F., Chinn I.K., Lupski J.R., Mace E.M., Cancrini C., Aiuti A., Ahmadian M.R., Orange J.S., De Benedetti F, & Tartaglia M. (2019). A novel disorder involving dyshematopoiesis, inflammation, and HLH due to aberrant CDC42 function. The Journal of Experimental Medicine, 216(12), 2778-2799.
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