Each sample was stained with Rhodamine B (0.01 g/100 mL water) for CLSM measurement in order to visualize protein microstructure. The dye was kneaded combined with the flour-water-system by replacing 5 mL of bulk water with Rhodamine B solution according to the bulk water technique [17 (link)]. An eclipse Ti-U inverted microscope with an e-C1 plus confocal system (Nikon GmbH, Düsseldorf, Germany) with a Plan Apo VC 60x/1.40 oil objective and a 534 nm laser (emission 590/50 nm) were used for visualization. Eight different images were taken of each sample with a resolution of 1024 × 1024 pixel and a size of 215 × 215 µm.
Plan apo vc 60x 1.40 oil objective
Manufactured by Nikon
Sourced in Germany
The Nikon Plan Apo VC 60x/1.40 oil objective is a high-performance objective lens designed for microscopy applications. It features a numerical aperture of 1.40 and a magnification of 60x, providing excellent optical performance and resolution. The lens is optimized for use with oil immersion, ensuring high image quality and contrast.
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Lab products found in correlation
2 protocols using plan apo vc 60x 1.40 oil objective
1
Visualizing Protein Microstructure with Rhodamine B
2
Confocal Microscopy Analysis of Gluten Proteins
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For the visualization of the gluten proteins by CLSM, all dough samples were stained with Rhodamine B (0.01 g/100 mL water) according to the bulk water technique17 (link). An eclipse Ti-U inverted microscope with an e-C1 plus confocal system (Nikon GmbH, Düsseldorf, Germany) with a Plan Apo VC 60x/1.40 oil objective and a 534 nm laser (emission 590/50 nm) were used for microstructure analysis. Eight independent (non-overlapping) images were taken of each sample with a resolution of 1024 × 1024 pixel and a size of 215 × 215 µm.
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