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Cyclin d1

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Cyclin D1 is a protein that plays a key role in the regulation of the cell cycle. It functions as a regulatory subunit of the cyclin-dependent kinase 4 (CDK4) enzyme, which is involved in the progression of cells through the G1 phase of the cell cycle.

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Lab products found in correlation

Cyclin a, by Santa Cruz Biotechnology (1 mentions) Antibodies against bcl 2, by Santa Cruz Biotechnology (1 mentions) Testosterone propionate, by Fujifilm (1 mentions) 5α reductase 2, by Bioneer (1 mentions) Cyclin e, by Santa Cruz Biotechnology (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Bcl xl, by Santa Cruz Biotechnology (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions) Finasteride, by Merck Group (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh oligonucleotide primers, by Bioneer (1 mentions) Nanog, by Bioneer (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Rna bee reagent, by Tel-Test (1 mentions) Maxima sybr green qpcr master mix, by Thermo Fisher Scientific (1 mentions)

3 protocols using cyclin d1

1

Molecular Mechanisms of Prostate Cancer Progression

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Testosterone propionate (Cat# 200-17532) was purchased from Wako Pure Chemicals (Tokyo, Japan). Finasteride was procured from Merck & Co., Inc. (NJ, USA). Antibodies against Bcl-2 (Cat# sc-7382), Bcl-xL (Cat# sc-8392), CDK2 (Cat# sc-748), CDK4 (Cat# sc-23896), Cyclin A (Cat# sc-751), Cyclin D1 (Cat# sc-753), Cyclin E (Cat# sc-481), E2F1 (Cat# sc-193), PCNA (Cat# sc-56), p-RB (Cat# sc-377528), and β-actin (Cat# sc-81178) were purchased from Santa Cruz biotechnology, Inc. (Dallas, TX, USA). Antibody against PSA (Cat# PB9259) was procured from Boster Biological Technology (Pleasanton, CA, USA). Horseradish peroxidase conjugated secondary antibodies (Cat# 315-035-003 and 111-035-003) were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). SYBR Green Master Mix (Cat# 4367659) was purchased from Applied Biosystems (Foster City, CA, USA). 5α-reductase 2, CDK2, CDK4, Cyclin A, Cyclin D1, Cyclin E, E2F1, PCNA, PSA, RB, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) oligonucleotide primers were purchased from Bioneer (Daejeon, Korea).
Jin B.R., Cheon S.Y., Kim H.J., Kim M.S., Lee K.H, & An H.J. (2020). Anti-Proliferative Effects of Standardized Cornus officinalis on Benign Prostatic Epithelial Cells via the PCNA/E2F1-Dependent Cell Cycle Pathway. International Journal of Molecular Sciences, 21(24), 9567.
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2

RNAi Knockdown of Pluripotency Genes

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Synthetic siRNAs specific for GFP, SCP3, NANOG were purchased from Bioneer; nonspecific GFP, 5′- GCAUCAAGGUGAACUUCAA-3′ (sense), 5′-UUGAAGUUCACCUUGAUGC-3′ (antisense); SCP3 #1 5′-CAGUUAUAUGAGCAGUUCAUAAA-3′ (sense); 5′- UUUAUGUUCUGCUCAUAUAACUG-3′ (antisense); SCP3 #2 5′-GAGACUAGAAAUGUAUACCAAGG-3′ (sense); 5′-CCUUGGUAUACAUUUCUAGUCUC-3′ (antisense); SCP3 #3 5′-GUGCAGAAUAUGCUGGAAGGAGU-3′ (sense); 5′-ACUCCUUCCAGCAUAUUCUGCAC-3′ (antisense); NANOG, 5′- GCAACCAGACCUGGAACAA-3′ (sense), 5′-UUGUUCCAGGUCUGGUUGC-3′ (antisense). For AKT (#001014431.1) and cyclin D1 (#053056.2), predesigned siRNAs were purchased commercially from Bioneer. siRNA was transfected in vitro into 6-well plates at a dose of 200 pmol per well using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
Oh S.J., Cho H., Kim S., Noh K.H., Song K.H., Lee H.J., Woo S.R., Kim S., Choi C.H., Chung J.Y., Hewitt S.M., Kim J.H., Baek S., Lee K.M., Yee C., Park H.C, & Kim T.W. (2018). Targeting Cyclin D-CDK4/6 Sensitizes Immune-Refractory Cancer by Blocking the SCP3–NANOG Axis. Cancer research, 78(10), 2638-2653.
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3

Resveratrol Modulates Cyclin D1 Expression in Huh7 Cells

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The Huh7 cells were seeded into 6-cm dishes at a density of 1.5 × 105 cells per dish, cultured in RPMI 1640 medium containing 10% FBS, and treated with resveratrol at varying concentrations. Total RNA was isolated by using RNA-Bee reagent (Tel-Test, USA) following the manufacturer's instructions, and cDNA was synthesized by using a reverse transcription system purchased from Promega (USA). The cDNA and specific primers were mixed with the Maxima SYBR Green qPCR master mix (Fermentas, Lithuania). The primer sequences were as follows: cyclin D1 (NM 007631.2), forward, 5′-agcagaagtgcgaagaggagg-3′ and reverse, 5′-ggcagtcaagggaa tggtctc-3′ and β-actin (X03672) forward, 5′-tgtccaccttccagca gatgt-3′ and reverse, 5′-agctcagtaacagtccgcctaga-3′. The primers were obtained from Bioneer (Korea).
Park S., Lim J., Kim J.R, & Cho S. (2017). Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma. Journal of Veterinary Science, 18(4), 419-429.
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